We narrowed to 11,635 results for: Vars

1. pUC19-E6MBW6

   Plasmid

   #103102

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   E6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
   

   Use

   CRISPR; Cloning vector

   Mutation

   human codon-optimized

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-6xHis-GFP(+36)-DL4

   Plasmid

   #199168

   Purpose

   used to express a supercharged GFP variant that forms inclusion bodies in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(+36)-DL4
   

   Tags

   MGHHHHHHGGA

   Mutation

   E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

   Promoter

   T7

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEThT-RvCAHS12

   Plasmid

   #192468

   Purpose

   Bacterial expression of RvCAHS12

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

   Insert

   CAHS12
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)

   Plasmid

   #184334

   Purpose

   Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

   Depositor

   Peter Scheiffele

   Article

   Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

   Insert

   Nrxn3-alpha (Nrxn3 Mouse)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSRW1JZ

   Plasmid

   #129528

   Purpose

   C. elegans codon-optimized FlincG3 (GFP-based cGMP sensor) expressed in C. elegans ASE neuron pair

   Depositor

   Noelle L'Etoile

   Article

   Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

   Insert

   FlincG3
   

   Expression

   Worm

   Available Since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. G718A_BattB(GA)_TKterm_AttB(GT)_sGFP-PTEN-IRES-mCherry-P2A-HygroR_noTerm_attP(GA)_IRES-UnaG-2A-Puro-Term

   Plasmid

   #200634

   Purpose

   Plasmid self-excising recombination plasmid for Matreyek Bxb1(GT) landing pad

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   PTEN (PTEN Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe EGFR Del2

   Plasmid

   #32065

   Depositor

   Matthew Meyerson

   Article

   Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

   Insert

   EGFR (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Delete S752-I759

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pEMS1996

   Plasmid

   #49126

   Purpose

   AAV plasmid with UGT8 (Ple267) promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-Ple267-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   UGT8

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCMV-dCas12a-A3B

   Plasmid

   #193642

   Purpose

   Expression of base editor dCas12a-A3B in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3B, EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-dCas12a-A3G

    Plasmid

    #193643

    Purpose

    Expression of base editor dCas12a-A3G in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-hA3G, EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_CRK-1/2

    Plasmid

    #91367

    Purpose

    Protein expression and purification of human SH3 domain construct CRK-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CRK-1/2 (CRK Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1P DDX11_2

    Plasmid

    #160778

    Purpose

    Suppress DDX11

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shDDX11_2
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1P DDX11_1

    Plasmid

    #160777

    Purpose

    Suppress DDX11

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shDDX11_1
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. sgRNA BRCA1 E638K

    Plasmid

    #139327

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 E638K using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 E638K using base editing
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Elafin

    Plasmid

    #18102

    Depositor

    Ronny Drapkin

    Article

    Labidi-Galy et al Oncogene. 2015 Jan 15;34(3):373-83. doi:

    Insert

    Elafin (PI3 Human)
    

    Expression

    Mammalian

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pMXS-IRES-Blast SHMT2res

    Plasmid

    #106301

    Purpose

    Expresses SHMT2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    serine hydroxymethyltransferase 2 (SHMT2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Silent mutations destroy sgRNA targeting sites

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR4 PARP2 E614Q

    Plasmid

    #192545

    Purpose

    pENTR plasmid Arabidopsis thaliana PARP2 CDS, E614Q mutation, no stop codon

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    poly(ADP-ribose) polymerase 2 E614Q (AT3G18250 Mustard Weed)
    

    Use

    Gateway entry vector

    Mutation

    changed Glu 614 to Gln

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSc-His6-Sem1Sc

    Plasmid

    #83235

    Purpose

    pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    Sem 1 (SEM1 Budding Yeast)
    

    Tags

    His6

    Expression

    Yeast

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. PF4V1-bio-His

    Plasmid

    #52073

    Purpose

    Expresses full-length Platelet factor 4 variant precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PF4V1 (PF4V1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEMS2157

    Plasmid

    #70118

    Purpose

    Promoterless AAV plasmid with MCS to insert promoter for expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-MCS-EmGFP-WPRE
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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