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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 5041 - 5060 of 11295 results

  5. MK01

    Bacterial Strain
    #195090
    Purpose
    Improved E. coli strain created by knocking out the endogenous lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783 that overcome the all-or-none response of araBAD promoter (PBAD).
    Depositor
    Sander Tans
    Article
    Kogenaru et al J Biol Eng. 2014 Jan 2;8(1):2. doi: 10.1
    Bacterial Resistance
    Chloramphenicol
    Available Since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only

  8. HOXA5 (human) HIS-tag pET

    Plasmid
    #8562
    Depositor
    Corey Largman
    Insert
    HOXA5 (HOXA5 Human)
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    SacI site at amino acids 1-2 was used to clone in…
    Available Since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  16. AviTag-SpyTag003-MBP

    Plasmid
    #184227
    Purpose
    Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    AviTag-SpyTag003-MBP
    Tags
    AviTag, His6, SpyTag003, and Thrombin cleavage si…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184227-plasmid-map-sequence-id-361606

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  18. pLTR.gp140/EGFP.Revdelta38/DsRed

    Plasmid
    #115775
    Purpose
    HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.
    Depositor
    Damian Purcell
    Article
    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37
    Insert
    Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
    Tags
    EGFP; dsReD
    Expression
    Mammalian
    Mutation
    gp140 uncleaved (1190-91aa, KR > TG; truncated…
    Promoter
    HIV-1 (LTR)
    Available Since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115775-plasmid-map-sequence-id-224438

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  19. PML-3/pSG5

    Plasmid
    #50939
    Purpose
    expression of PML with Sumo sites destroyed
    Depositor
    Pier Pandolfi
    Article
    Zhong et al Blood. 2000 May 1;95(9):2748-52.
    Insert
    PML-3 (PML Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    K65R, K160R, K490R (Sumo modification sites)
    Promoter
    SV40
    Available Since
    Jan. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 5041 - 5060 of 11295 results