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pBABE puro Brca1 S1423A S1524A HA

Plasmid

#41968

Depositor

Stephen Elledge

Article

Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

Insert

BRCA1 (BRCA1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

S1423A, S1524A

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3 AU1 mTOR S2035W

Plasmid

#15678

Depositor

John Lawrence

Article

McMahon et al Mol Cell Biol. 2002 Nov . 22(21):7428-38

Insert

mTOR (Mtor Rat)

Tags

AU1

Expression

Mammalian

Mutation

S2035W

Available Since

Sept. 24, 2007

Availability

Academic Institutions and Nonprofits only
pJW1510

Plasmid

#62364

Purpose

pFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-mVenus-Ubc6 tail anchor

Expression

Yeast

Mutation

L232H mutation compared to standard mVenus

Promoter

Sec63

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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Grp1-PH pGEX2TK

Plasmid

#71379

Purpose

bacterial expression of PH domain for phosphoinositide binding assay

Depositor

Mark Lemmon

Article

Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

Insert

Grp1-PH (Cyth3 Mouse)

Tags

GST

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 264-391

Promoter

tac

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pGEX2T merlin 1-332

Plasmid

#11631

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

merlin (NF2 Human)

Tags

GST

Expression

Bacterial

Mutation

Amino-terminal half of merlin.

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pGEX4T NHE-RF

Plasmid

#11636

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

NHE-RF (NHERF1 Human)

Tags

GST

Expression

Bacterial

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pAS163

Plasmid

#194486

Purpose

synMR Cassette

Depositor

Ahmad Khalil

Article

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synMR Cassette

Use

Synthetic Biology

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAS161

Plasmid

#194484

Purpose

synER Cassette

Depositor

Ahmad Khalil

Article

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synER Cassette

Use

Synthetic Biology

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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597 pCSmCherry

Plasmid

#31165

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

mCherry

Use

Expression vector

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pCSegfpN5ICD

Plasmid

#22467

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

Notch3 (notch3 Zebrafish)

Use

Injection construct

Available Since

March 8, 2010

Availability

Academic Institutions and Nonprofits only
CMV:AsCpf1-2A-GFP-U6-sgRNA-cloning vector

Plasmid

#194715

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that can be cloned with BbsI sites

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-AAVS1_sgRNA

Plasmid

#183890

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3_Cas7-11_T2A_Csx29

Plasmid

#197607

Purpose

Expresses Cas7-11 and Csx29 in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

Cas7-11_T2A_Csx29

Expression

Mammalian

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant mNG-ORP8

Plasmid

#195170

Purpose

mammalian expression of siRNA-resistant ORP8 tagged with mNeonGreen

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ORP8 (OSBPL8 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from K174 t…

Promoter

CMV

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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1099-N-cadherin-AAA-YFP

Plasmid

#32237

Depositor

Kathleen Green

Article

Chen et al J Cell Biol. 2003 Nov 10;163(3):547-57.

Insert

N-cadherin (Cdh2 Mouse)

Tags

EYFP

Expression

Mammalian

Mutation

Glu–Glu–Asp (aa 780–782) ---> Ala–Ala–Ala

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
pFB2

Plasmid

#195282

Purpose

Bicistronic DTR and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

DTR 2A Puro (HBEGF Human, Synthetic)

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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VAMP2-SNAP

Plasmid

#105288

Purpose

VAMP2 tagged with SNAP

Depositor

Justin Taraska

Article

Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

Insert

VAMP2-SNAP (Vamp2 Rat)

Tags

SNAP tag

Expression

Mammalian

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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PresentER-MSIIFFLPL (GFP)

Plasmid

#102946

Purpose

PresentER minigene encoding H2-Kb ligand MSIIFFLPL (GFP)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Insert

MSIIFFLPL (Serpinf1 Synthetic, Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SAMTOR

Plasmid

#100513

Purpose

Expresses HA tagged SAMTOR in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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U6>sgRNA(F+E)

Plasmid

#59986

Purpose

Empty vector for sgRNA cloning (F+E modified backbone)

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Type

Empty backbone

Use

CRISPR

Promoter

Ciinte.U6

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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