We narrowed to 13,832 results for: Cas9

1. LBJJ382

   Plasmid

   #117518

   Purpose

   Also known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminator

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26

   Available since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMiniT:SEP-GluA3 TKIT donor

   Plasmid

   #169425

   Purpose

   Donor DNA for TKIT knockin of SEP to GluA3 in mouse

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-U6-TTLL11

   Plasmid

   #140582

   Purpose

   Expresses a gRNA and mCherry in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   gRNA TTLL11
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEVA231-CRISPR

   Plasmid

   #138712

   Purpose

   Plasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR Array

   Depositor

   Víctor de Lorenzo

   Article

   Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SK-YFP-SPNM-B

   Plasmid

   #48661

   Purpose

   Bacterial SP and NM repression YFP reporter: protospacer B

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   SP/NM prototspacer B/PAM with reporter EYFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   lambda pR promoter

   Available since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. Hulk-ubi-lacZ-to-Green

   Plasmid

   #108641

   Purpose

   ubiquitous promoter followed by a lacZ to GFP lineage tracing line. Cryst:venusGFP

   Depositor

   Nadia Mercader Huber

   Article

   Di Donato et al Genome Res. 2016 Mar 8.

   Insert

   ubi-lacZ-to-GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB8381

   Plasmid

   #126915

   Purpose

   Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and tHMG1

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   XdCrtI and tHMG1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR1054

   Plasmid

   #71822

   Purpose

   Express Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLS

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCherry-U6-CLIC3

   Plasmid

   #140583

   Purpose

   Expresses a gRNA and mCherry in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   gRNA CLIC3
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-pegRNA-HEK3+24

    Plasmid

    #140577

    Purpose

    Expresses a pegRNA in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    pegRNA HEK3 +24
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. peft-3::cas-9::tbb-2 3'UTR

    Plasmid

    #48960

    Purpose

    this plasmid contains the codon optimized gene encoding Cas9 for C. elegans.

    Depositor

    Mario de Bono

    Article

    Chen et al Nucleic Acids Res. 2013 Sep 5.

    Insert

    cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR1003

    Plasmid

    #71821

    Purpose

    Express Streptococcus pyogenes dCas9 (D10A, H840A)

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Insert

    T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJin-278

    Plasmid

    #125714

    Purpose

    ABA-inducible split T7 GFP vector using RNAPN d5-19

    Depositor

    Bryan Dickinson

    Article

    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

    Insert

    PT7-GFP mRNA expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOsU3-esgRNA

    Plasmid

    #115629

    Purpose

    expression esgRNA in rice protoplasts

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    OsU3p-esgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG ERT2-Cre-ERT2 lox

    Plasmid

    #183447

    Purpose

    CreOFF tamoxifen-inducible Cre

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    ERT2-Cre-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB8792

    Plasmid

    #126910

    Purpose

    Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    ADE2 gRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. BCJJ339

    Plasmid

    #117501

    Purpose

    RPS5a:Cas9:E9 Golden Gate Level 1 Position 2

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:GCAA:RPS5a:Cas9-3(intron):E9:ACTA:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtRPS5a

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNeurog2-Luciferase reporter

    Plasmid

    #64126

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of the Neurog2 promoter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    Neurog2 promoter
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Neurog2

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19, containing H2-Kb c57/BL6 WK9

    Plasmid

    #102641

    Purpose

    Encodes sequence of the murine H2-Kb allele

    Depositor

    Sai Reddy

    Article

    Kelton et al Sci Rep. 2017 Apr 4;7:45775. doi: 10.103

    Insert

    H2-Kd Balb/c
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmCherry-U6-EMX1

    Plasmid

    #140581

    Purpose

    Expresses a gRNA and mCherry in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    gRNA EMX1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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