We narrowed to 12,101 results for: VARS

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pUG-natNT2

Plasmid

#110922

Purpose

expression of nat gene conferring resistance to nourseothricin for gene deletion in yeast

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

nat

Expression

Bacterial and Yeast

Promoter

AgTEF1

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pME map2k2a_s219D

Plasmid

#201058

Purpose

Gateway middle entry vector expressing constitutively active zebrafish map2k2a

Depositor

Sarah Childs

Article

Greysson-Wong et al Development. 2023 Sep 15;150(18):dev2018

Insert

MAP2k2a (map2k2a Zebrafish)

Use

Zebrafish expression

Mutation

Mutation S219D, constitutively active

Promoter

none

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 P.1

Plasmid

#173476

Purpose

Encodes SARS-CoV-2 P.1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 Spike Truncated to remove 18 amino acids

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCK341

Plasmid

#192640

Purpose

Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Inserts

Sp.pCas9-dSpRY
BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR and Synthetic Biology

Mutation

SoxS has R93A and S101A mutations and dSpRY has s…

Promoter

BBa_J23107 and Sp.pCas9

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_CTTN-1/1

Plasmid

#91550

Purpose

Protein expression and purification of human SH3 domain construct CTTN-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CTTN-1/1 (CTTN Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_linker

Plasmid

#211731

Purpose

Linker fragment for direct rescue of SARS-CoV-2, including 5' Promoter and 3' Termination signals and 100bp SARS-CoV-2 homology regions

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

Linker fragment SARS-CoV-2

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-MPRA-MluI-SpeI-EcoRI

Plasmid

#190196

Purpose

Empty vector for inserting MPRA libraries into AAV

Depositor

Hyejung Won

Article

McAfee et al Cell Genom. 2023 Sep 15;3(10):100404. do

Type

Empty backbone

Use

AAV; Massively parallel reporter assay (mpra)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57-CMV-MAAP2

Plasmid

#218244

Purpose

1) Ectopic expression of MAAP2 protein in mammalian cells. 2) Packaging MAAP2 sequence into lentivirus for stable expression in mammalian cells.

Depositor

David Schaffer

Article

Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

Insert

Membrane-Associated Accessory Protein from Adeno-Associated Virus 2 driven by CMV promoter

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR(L861Q)

Plasmid

#32068

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

L861Q

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pHH0103_GAS7-1/1

Plasmid

#91301

Purpose

Protein expression and purification of human SH3 domain construct GAS7-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GAS7-1/1 (GAS7 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_S139A-GS3

Plasmid

#204749

Purpose

Expression of Cas9 and human H2AX with the S139A mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX S139A (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the S139A mutation

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-CreERT2 sgRipk4 v3

Plasmid

#158048

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Ripk4. NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Ripk4 sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2232

Plasmid

#158401

Purpose

High Copy number plasmid containing Ple316 (NR2E1 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple316 (NR2E1 Human)

Use

Unspecified

Promoter

Ple316

Available Since

Feb. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pT2/sh-Col1a1/GFP4_Seq1.2

Plasmid

#201403

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-CreERT2 sgAdam10 v3

Plasmid

#158047

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Adam10. NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Adam10 sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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ScSpe1 pET-20b(+)

Plasmid

#117145

Purpose

Expresses the His tagged Saccharomyces cerevisiae Ornithine Decarboxylase in Escherichia coli BL21

Depositor

Markus Ralser

Article

Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

Insert

SPE1

Tags

Histidine

Expression

Bacterial

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT105

Plasmid

#180510

Purpose

Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Inserts

CasX sgRNAv2
DpbCasX with R3 loop

Use

CRISPR

Tags

2x FLAG and SV40 NLS

Expression

Mammalian

Promoter

CAG and U6

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

Plasmid

#139086

Purpose

Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

SpCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3A

Plasmid

#193638

Purpose

Expression of base editor dCas12a-A3A in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro human-GFP-N581K RLIM-IRES-mCherry

Plasmid

#232244

Purpose

Mammalian expression of N581K RLIM-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

N581K RLIM-GFP-IRES-mCherry (RLIM Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

N581K

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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