Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 63,812 results for: cloning

Clear keywords
Showing: 5061 - 5080 of 63812 results

  7. DCT.VV.Orf107

    Plasmid
    #12795
    Purpose
    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.
    Depositor
    Jack Bennink, Jon Yewdell
    Publication
    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VV.Orf107
    Expression
    Mammalian
    Available since
    Dec. 8, 2006
    Availability
    Academic Institutions and Nonprofits only

  10. pGTM_YR375_SUMO_Protease_001

    Plasmid
    #190063
    Purpose
    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion
    Depositor
    Gaetano Montelione
    Publication
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    ULP1_YEAST (ULP1 Budding Yeast)
    Tags
    8X His-MBP (Maltose Binding Protein)
    Expression
    Bacterial
    Mutation
    Construct contains residues 347 – 621
    Promoter
    T7 lac
    Available since
    Jan. 30, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    190063-plasmid-map-sequence-id-370664

    Enlarge

  11. OpenCRISPR-1 sgRNA-12nt stem

    Plasmid
    #221567
    Purpose
    Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.
    Depositor
    Ali Madani
    Publication
    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OpenCRISPR-1 sgRNA 12nt stem loop
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 28, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221567-plasmid-map-sequence-id-437907

    Enlarge

  18. iF59 PB-TO-oNgn2-puro

    Plasmid
    #204734
    Purpose
    Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Insert
    Optimized neurogenin-2
    Expression
    Mammalian
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    204734-plasmid-map-sequence-id-403386

    Enlarge

  20. pEF1a-PEmax-P2A-GFP

    Plasmid
    #202000
    Purpose
    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.
    Depositor
    Daniel Geschwind
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEmax-P2A-GFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    202000-plasmid-map-sequence-id-395506

    Enlarge

Showing: 5061 - 5080 of 63812 results