Addgene

We narrowed to 6,563 results for: mag

Showing: 5061 - 5080 of 6563 results

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mCardinal-Actin-C-18

Plasmid

#56151

Purpose

Localization: Actin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

NM_001101.3 (ACTB Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL

Plasmid

#63885

Purpose

An AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized low-affinity GCaMP3(10.19)

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His K1278R CRres si2,4R

Plasmid

#139406

Purpose

Dox-inducible, K1278R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

K1278R

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pRepair-SGFP2-CTNNB1

Plasmid

#153430

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

SGFP2

Expression

Mutation

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pX459-CTNNB1-ATG

Plasmid

#153429

Purpose

Expresses a gRNA that overlaps the startcodon of human CTNNB1

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 gRNA (CTNNB1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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mCardinal-MCAK-C-7

Plasmid

#56168

Purpose

Localization: Microtubules/Kinesin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

MCAK (KIF2C Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pEX-YFP-STIM1-deltaK

Plasmid

#18861

Depositor

Tobias Meyer

Article

Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 1

Insert

STIM1 (STIM1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Stop codon inserted at amino acid postion 671 of …

Promoter

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pDB-hCRY1-Halo-CD4-bla

Plasmid

#179445

Purpose

Donor vector to knock in Halotag C-terminal to human CRY1 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

HaloTag (Halo7 Synthetic)

Use

CRISPR

Tags

His/Flag

Expression

Mutation

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-puro/Flag PD-L1 4NQ

Plasmid

#121456

Purpose

expression of human PD-L1 mutant in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-jYCaMP1

Plasmid

#135422

Purpose

Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection

Depositor

Kaspar Podgorski

Article

Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

Insert

jYCaMP1

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFV2-mVenus

Plasmid

#185653

Purpose

NDUFV2 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFV2 (NDUFV2 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-jYCaMP1s

Plasmid

#135420

Purpose

Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

Depositor

Kaspar Podgorski

Article

Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

Has Service

AAV1

Insert

jYCaMP1s

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-Rosmarinus

Plasmid

#183462

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-rosmarinus fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

Rosmarinus

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFS6-mVenus

Plasmid

#185806

Purpose

NDUFS6 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFS6 (NDUFS6 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRepair-SYFP2-CTNNB1

Plasmid

#153432

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

SYFP2

Expression

Mutation

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEX-CFP-STIM1-deltaK

Plasmid

#18860

Depositor

Tobias Meyer

Article

Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 1

Insert

STIM1 (STIM1 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Stop codon at amino acid position 671 of STIM1. C…

Promoter

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-mCarmine

Plasmid

#183464

Purpose

To produce AAV with the P3 promoter to express Alb-mCarmine fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mCarmine

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFA7-mVenus

Plasmid

#185647

Purpose

NDUFA7 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA7 (NDUFA7 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-RNF168 delta MIU1

Plasmid

#133978

Purpose

mammalian expression vector of Flag tagged RNF168 where the first motif interacting with ubiquitin (MIU) has been deleted

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deletion of MIU1

Promoter

CMV

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-6P-1-HRD1 aa 236-444

Plasmid

#185349

Purpose

RING domain-containing GST fusion protein for ubiquitination assays.

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

HRD1/SYVN (SYVN1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 236-444 only

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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