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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 5061 - 5080 of 6562 results

  1. pCAG-T3-SpCAS9-NG-pA

    Plasmid
    #131465
    Purpose
    Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Publication
    Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized SpCas9-NG
    Use
    CRISPR
    Tags
    Flag, NLS, and polyA
    Expression
    Mammalian
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131465-plasmid-map-sequence-id-262447

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  7. pcDNA-Flag-EcoCas8e

    Plasmid
    #106270
    Purpose
    Encodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EcoCasA
    Use
    CRISPR
    Tags
    Flag, NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    106270-plasmid-map-sequence-id-246604

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  8. pcDNA-Flag-EcoCse2

    Plasmid
    #106271
    Purpose
    Encodes human codon optimized E.coli CRISPR Type I-E CasB with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EcoCasB
    Use
    CRISPR
    Tags
    Flag, NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    106271-plasmid-map-sequence-id-246603

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  9. pcDNA-Flag-EcoCas7

    Plasmid
    #106272
    Purpose
    Encodes human codon optimized E.coli CRISPR Type I-E CasC with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EcoCasC
    Use
    CRISPR
    Tags
    Flag, NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    106272-plasmid-map-sequence-id-246605

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  10. pcDNA-Flag-EcoCas5

    Plasmid
    #106273
    Purpose
    Encodes human codon optimized E.coli CRISPR Type I-E CasD with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EcoCasD
    Use
    CRISPR
    Tags
    Flag, NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    106273-plasmid-map-sequence-id-246606

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  11. pcDNA-Flag-EcoCas6

    Plasmid
    #106274
    Purpose
    Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EcoCasE
    Use
    CRISPR
    Tags
    Flag, NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    106274-plasmid-map-sequence-id-246607

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  12. pKG194

    Plasmid
    #69757
    Purpose
    Mammalian Expression of CENP-T aa 1-375
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-375 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and eGFP
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Available since
    Nov. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. PX395 Roseburia intestinalis Cas9

    Plasmid
    #68332
    Purpose
    Roseburia intestinalis Cas9
    Depositor
    Feng Zhang
    Insert
    RiCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68332-plasmid-map-sequence-id-119548

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  17. pYPQ284-v2

    Plasmid
    #138119
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R, N563R and K569R mutations, without promoter
    Depositor
    Yiping Qi
    Publication
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    Mb2Cas12a v2
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    D172R, N563R and K569R
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138119-plasmid-map-sequence-id-272076

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  18. pYPQ233-STU

    Plasmid
    #138111
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated LbCas12a-SRDX repressor without promoter and terminator
    Depositor
    Yiping Qi
    Publication
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    dLbCas12a-SRDX
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138111-plasmid-map-sequence-id-272043

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Showing: 5061 - 5080 of 6562 results