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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 37,196 results for: ELL

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Showing: 5081 - 5100 of 37196 results

  1. pDS240

    Plasmid
    #34966
    Purpose
    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).
    Depositor
    Michael Glotzer
    Article
    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm
    Insert
    Mid2(SS/TM)-GFP-LOVpep
    Expression
    Yeast
    Mutation
    K533∆, high-affinity epitope
    Promoter
    GAL1
    Available Since
    March 21, 2012
    Availability
    Academic Institutions and Nonprofits only
    34966-plasmid-map-sequence-id-42500

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  10. DBDmut-CA-RIT-NFAT1

    Plasmid
    #63669
    Purpose
    Constitutively active NFAT1 and also unable to interact with AP-1 with four point mutations in the DNA binding loop that abolish DNA binding
    Depositor
    Anjana Rao
    Article
    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15
    Insert
    NFAT1 (Nfatc2 Mouse)
    Use
    Retroviral
    Tags
    3x HA
    Expression
    Mammalian
    Mutation
    CA: PASSGSSASF mutated to PAAAGAAAAF, SPRTSPIMSP…
    Available Since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

    Plasmid
    #49077
    Purpose
    Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x HA and mCerulean
    Expression
    Mammalian
    Mutation
    262 silent mutations from native hNKCC1
    Promoter
    CMV
    Available Since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    49077-plasmid-map-sequence-id-72205

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  13. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV8)

    Viral Prep
    #112677-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Article
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available Since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 5081 - 5100 of 37196 results