168,922 results

1. pcDNA3.1-HaloAKAR2.2-T2A-EGFP

   Plasmid

   #216490

   Purpose

   Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAKAR2.2
   

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIS1

   Plasmid

   #12179

   Depositor

   David Bartel

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ub-G76V-GFP

   Plasmid

   #11941

   Depositor

   Nico Dantuma

   Article

   Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

   Insert

   Ub-G76V-GFP
   

   Expression

   Mammalian

   Mutation

   Ubiquitin fused to N-terminus of GFP. Glycine 76 …

   Available Since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR SFFVp PHAkt Cerulean

   Plasmid

   #50837

   Purpose

   Fluorescent reporter for membrane PIP3 levels

   Depositor

   Wendell Lim

   Article

   Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

   Insert

   PHAkt (AKT1 )
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Cerulean

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMpGWB309

   Plasmid

   #68637

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   3xFLAG

   Expression

   Plant

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST/hCx43-EGFP-N1

   Plasmid

   #40907

   Depositor

   Robin Shaw

   Article

   Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

   Insert

   Connexin 43 (GJA1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   No stop codon to create c-terminal fusion

   Promoter

   CMV

   Available Since

   Nov. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIP-Flag-TfebAA

   Plasmid

   #79014

   Purpose

   For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Tfeb (Tfeb Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S142A, S211A

   Promoter

   pCAGGs

   Available Since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-mCardinal

   Plasmid

   #51311

   Purpose

   Expresses far-red fluorescent protein mCardinal in mammalian cells

   Depositor

   Michael Lin

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   mCardinal
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-FLEX-TeLC-P2A-dTomato

   Plasmid

   #159102

   Purpose

   Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

   Depositor

   Sandeep Datta

   Article

   Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

   Insert

   TeLC-P2A-NLS-dTomato
   

   Use

   AAV

   Promoter

   hSynapsin

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. PCMV4-3 HA/IkB-alpha(SS32,36AA)

    Plasmid

    #24143

    Depositor

    Warner Greene

    Article

    Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

    Insert

    IkB alpha (NFKBIA Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    S32A S36A

    Available Since

    March 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTagRFP-C1-Fibrillarin

    Plasmid

    #70649

    Purpose

    Expresses Fibrillarin nucleolar marker with TurboRFP reporter.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    Fibrillarin (FBL Human)
    

    Tags

    TagRFP

    Expression

    Mammalian

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. PGK-H2BmCherry

    Plasmid

    #21217

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Phosphoglycerate kinase promoter (PGK1 Human)
    

    Use

    Lentiviral

    Tags

    H2B-mCherry

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDx_mScarlet3-SYFP2

    Plasmid

    #189764

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-SYFP2
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid

    #73797

    Purpose

    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-C-mStayGold-EFS-Puro

    Plasmid

    #227256

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. ER-B

    Plasmid

    #209870

    Purpose

    Dimerization dependent fluorescent protein B anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Tags

    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC18T-mini-Tn7T-Gm-dsRedExpress

    Plasmid

    #65032

    Purpose

    GmR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    dsRedExpress
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pcDNA3.1-ERBB2-V5/HIS

    Plasmid

    #201103

    Purpose

    expression of human ERBB2 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human ERBB2 receptor tyrosine kinase, full length, wildtype (ERBB2 Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAS134

    Plasmid

    #211505

    Purpose

    AAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

    Depositor

    Randall Platt

    Article

    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

    Insert

    Venus
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Expression

    Mammalian

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR

    Plasmid

    #210523

    Purpose

    AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    GFP-KASH
    U6-gRNA-directcapture
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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