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p5073 pcDNA4c hBrd4 (1047-1223)

Plasmid

#14452

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1047-1223) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1047-1223

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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p5081 pcDNA4c hBrd4 (1134-1223)

Plasmid

#14457

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1134-1223) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1134-1223

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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COL6A2_pCSdest

Plasmid

#53903

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

COL6A2 (COL6A2 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pQCascade-IS186

Plasmid

#140627

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS186

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2438 - [1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159862

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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ATP5O_pCSdest

Plasmid

#53812

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ATP5O (ATP5PO Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
HOXA11 (murine) HIS-tag pET

Plasmid

#8517

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA11 (Hoxa11 Mouse)

Tags

T7

Expression

Bacterial

Mutation

makes protein by Western

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT TO myc Separase C2029A

Plasmid

#59822

Purpose

Allows the integration of myc Separase C2029A in the genome and Tet-inducible expression

Depositor

Stephen Taylor

Article

Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

C2029A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pAf-CRISPR-phoA

Plasmid

#207991

Purpose

CRISPR vector used with pAf-CRISPR-ctfR1 (#207992) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

Depositor

Perng-Kuang Chang

Article

Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

Insert

phoA

Use

CRISPR

Promoter

Aspergillus flavus U6

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD4 Flag

Plasmid

#74896

Purpose

Express COMMD4 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD4 (COMMD4 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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BACE2_pCSdest

Plasmid

#53915

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

BACE2 (BACE2 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pEBB COMMD7 Flag

Plasmid

#74899

Purpose

Express COMMD7 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD7 (COMMD7 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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CXADR_pCSdest

Plasmid

#53853

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

CXADR (CXADR Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pSH231-EF1-BLST-Cas9-VPR

Plasmid

#115147

Purpose

Safe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

BlastR-P2A-Cas9-VPR

Use

CRISPR

Tags

SV40NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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LSS_pCSdest

Plasmid

#53931

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

LSS (LSS Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pMRE-Tn5-151

Plasmid

#118533

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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p4979 pcDNA4-E6AP III L502P

Plasmid

#32892

Depositor

Peter Howley

Article

Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

Insert

E6AP (UBE3A Human)

Tags

6xHis Express and T7-tag

Expression

Mammalian

Mutation

L502P

Promoter

CMV

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only
p4978 pcDNA4-E6AP III E550L

Plasmid

#32891

Depositor

Peter Howley

Article

Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

Insert

E6AP (UBE3A Human)

Tags

6xHis Express and T7-tag

Expression

Mammalian

Mutation

E550L

Promoter

CMV

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only
pDECKO-mCherry MALAT1_Enhancer.1

Plasmid

#78536

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA) and U6 promoter

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCA-T-intron(Neo)-G(LNL)

Plasmid

#36908

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

T (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Neo

Expression

Mammalian

Mutation

deleted nucleotides before nucleotide 275 and ins…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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