We narrowed to 17,432 results for: she

1. mT2A-tdTomato-FNF

   Plasmid

   #182340

   Purpose

   Recombineering cassette plasmid for T2A-tdTomato-FNF

   Depositor

   Mario Capecchi

   Insert

   T2A-tdTomato-FNF
   

   Use

   Mouse Targeting; Flp / frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22B_PcrA_2Bchimera

   Plasmid

   #102998

   Purpose

   Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA

   Depositor

   Martin Webb

   Insert

   PcrA_2Bchimera
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Bacilllus stearothermophilus PcrA in which the 2B…

   Promoter

   T7

   Available since

   May 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCP-tag (m) Vector

   Plasmid

   #101127

   Purpose

   Mammalian expression plasmid that allows for the expression of a gene of interest fused to the N-terminus or C-terminus of MCP-tag.

   Depositor

   Ana Egana, New England Biolabs

   Type

   Empty backbone

   Use

   Tags

   MCP-tag (Mutant ACP- tag)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pHD-w+

   Plasmid

   #80927

   Purpose

   Vector for generating dsDNA donors for HDR. Empty backbone containing a StuI restriction site and GMR-white for identifying backbone incorporation.

   Depositor

   Kate O'Connor-Giles

   Insert

   GMR-white
   

   Use

   High copy, amp resistance

   Tags

   Expression

   Mutation

   Promoter

   GMR::hsp70

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.3_SARS2_EG.5.1

   Plasmid

   #207641

   Purpose

   pseudovirus plasmid that expresses spike of EG.5.1

   Depositor

   David Nemazee

   Insert

   Spike (EG.5.1) (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q52H, F456L on the basis of XBB.1.5

   Promoter

   CMV

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEos3.2-Rad21-N

   Plasmid

   #66951

   Purpose

   Localization: Centromere, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson

   Insert

   Rad21 (RAD21 Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pBEST-PLtetO-1-UTR1-TetR-deGFP-T500

   Plasmid

   #45774

   Depositor

   Richard Murray

   Insert

   tetR-deGFP
   

   Use

   Synthetic Biology

   Tags

   Fusion protein of TetR and deGFP, with glycine-se…

   Expression

   Bacterial

   Mutation

   Promoter

   PLtetO-1

   Available since

   July 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9CR4-NG

   Plasmid

   #120225

   Purpose

   pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.

   Depositor

   Christopher Reisch

   Insert

   SpCas9-NG
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Expression

   Mutation

   Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…

   Promoter

   pTet

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUS252L

   Plasmid

   #191832

   Purpose

   Expresses fuBFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuBFP to place in other plasmid backbones

   Depositor

   Nicholas Coleman

   Insert

   fuBFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pet15_rbsk

    Plasmid

    #220227

    Purpose

    Bacterial expression of Rbsk in E. coli BL21_DE3 (pLys) or similar

    Depositor

    Bernd Nidetzky

    Insert

    ribokinase (rbsK E.coli)
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK651

    Plasmid

    #71702

    Purpose

    Produces E. coli thioredoxin reductase with a C-terminal His6 tag (EcTrxBH6)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin reductase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV2-U6(BsmBI)-EF1a-BFP-BSD

    Plasmid

    #117136

    Purpose

    Empty vector encoding BFP-BSD

    Depositor

    Kosuke Yusa

    Insert

    BSD2A
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLIK 3xFLAG-NUP37 neo

    Plasmid

    #220361

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-tagged NUP37

    Depositor

    Kevin Janes

    Insert

    NUP37 (NUP37 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHflu2

    Plasmid

    #203880

    Purpose

    pHI1 with insertion of ampicillin-resistance gene

    Depositor

    Bogumil Karas

    Insert

    broad-spectrum class A beta-lactamase TEM-116
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEos3.2-Vinculin-N-21

    Plasmid

    #66952

    Purpose

    Localization: Focal Adhesions, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Michael Davidson

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    V234L in Vinculin

    Promoter

    CMV

    Available since

    July 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pMCP-GPI Control Plasmid

    Plasmid

    #101132

    Purpose

    Expression of MCP-Tagged GPI anchor in Mammalian cells

    Depositor

    Ana Egana, New England Biolabs

    Insert

    GPI
    

    Use

    Tags

    MCP-tag (Mutant ACP- tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. miR-124

    Plasmid

    #182363

    Purpose

    Plasmid with 4x miR-124 target sites

    Depositor

    Mario Capecchi

    Insert

    4x miR-124 target sites
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpCas9_hGEM(1-110)_hSLBP(56-108)

    Plasmid

    #191388

    Purpose

    SpCas9 fused with geminin and SLBP degrons to improve HDR ratio

    Depositor

    Yannick Doyon

    Insert

    hGEM(1-110)_hSLBP(56_108)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-H2B-sfGFP-T2A-iCre

    Plasmid

    #183351

    Purpose

    Histone H2B-sfGFP and iCre recombinase

    Depositor

    Mario Capecchi

    Insert

    Histone H2B-sfGFP-T2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. p221-RV-dlx-dio-Tdtomato

    Plasmid

    #185697

    Purpose

    Retroviral vector expression cre-dependent membrane tagged Tdtomato driven by the mDlx promoter

    Depositor

    Lynette Lim

    Insert

    membrane Tdtomato
    

    Use

    Cre/Lox and Retroviral

    Tags

    Gap43 palmitoylation

    Expression

    Mutation

    Promoter

    mDlx

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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