Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 9,544 results for: pho;

Showing: 7681 - 7710 of 9544 results

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pLenti-U6-DCK-Hygro

Plasmid

#202409

Purpose

gRNA expression vector for DCK

Depositor

Morito Kurata

Article

Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

Insert

guide RNA (DCK Human)

Use

CRISPR and Lentiviral

Tags

Dck

Promoter

U6

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-bio-myc-mCE 211-597

Plasmid

#112716

Purpose

Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 211-597 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Tags

biotinylation signal peptide (Tagwerker et al. 20…

Expression

Mammalian

Mutation

deleted amino acids 2-210 (triphosphatase domain)

Promoter

CMV

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pEB1N-mApple-LZ-LOV2

Plasmid

#197418

Purpose

Mammalian expression of the mApple-tagged N-terminal half of photo-inactivated π-EB1

Depositor

Torsten Wittmann

Article

Dema et al Curr Biol. 2022 Mar 14;32(5):1197-1205.e

Insert

MAPRE1 (MAPRE1 Human)

Tags

A. sativa phototropin 1 LOV2 domain, GCN4 leucine…

Expression

Mammalian

Mutation

EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-ENO1_sgRNA1

Plasmid

#201592

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

ENO1 (ENO1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-ENO1_sgRNA2

Plasmid

#201593

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

ENO1 (ENO1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-HNRNPD_sgRNA2

Plasmid

#201599

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

HNRNPD (HNRNPD Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-LUC7L_sgRNA1

Plasmid

#201600

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L (LUC7L Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-LUC7L_sgRNA2

Plasmid

#201601

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L (LUC7L Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-LUC7L3_sgRNA1

Plasmid

#201604

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L3 (LUC7L3 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-LUC7L3_sgRNA2

Plasmid

#201605

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L3 (LUC7L3 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-PABPC1_sgRNA2

Plasmid

#201609

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PABPC1 (PABPC1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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PGK EGFP-LC3B

Plasmid

#200427

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

EGFP

Expression

Mammalian

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-hMIGA2 (170-593)

Plasmid

#192833

Purpose

Express codon-optimized human MIGA2 fragment 170-593 in Expi293 cells

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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plx307 SHOC2 V5 Q249K

Plasmid

#190570

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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plx307 SHOC2 WT G63R

Plasmid

#190569

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
plx307 SHOC2 V5 F66V

Plasmid

#190571

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
plx307 SHOC2 V5 E457K

Plasmid

#190572

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
plx307 SHOC2 V5 Y131E

Plasmid

#190573

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
plx307 SHOC2 V5 M173I

Plasmid

#190574

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
plx307 SHOC2 V5 M173V

Plasmid

#190575

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
plx307 SHOC2 V5 R223F

Plasmid

#190576

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
plx307 SHOC2 V5 T411A

Plasmid

#190577

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
plx307 SHOC2 V5 V64G

Plasmid

#190578

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
plx307 SHOC2V5 N434D

Plasmid

#190579

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
plx307 SHOC2 V5 R182E

Plasmid

#190580

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
plx307 SHOC2 V5 M219V

Plasmid

#190581

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUGW-shRIIα-RIIαS97A-IRES-EGFP

Plasmid

#183457

Purpose

Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUGW-shRIIα-RIIαS97E-IRES-EGFP

Plasmid

#183458

Purpose

Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA T7-Gnat1 Mut-3UTR

Plasmid

#184027

Purpose

Expresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGA

Depositor

Peter Stoilov

Article

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Gnat1 (Gnat1 Mouse)

Tags

T7

Expression

Mammalian

Mutation

TAG sites in the 3'-UTR are mutated to TGA

Promoter

CMV

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D

Plasmid

#175051

Purpose

Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunit 5 (RFC2, RFC3, RFC4, RFC5 Human)

Expression

Bacterial

Mutation

changed Thr76 to Asp, phosphomimetic mutation

Promoter

T7 promoter

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 7681 - 7710 of 9544 results

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