We narrowed to 166,096 results for: addgene

1. TNFa-GFP

   Plasmid

   #163660

   Purpose

   Expresses GFP tagged TNFa in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   tumor necrosis factor (TNF Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDYH5

   Plasmid

   #52032

   Purpose

   Expression of GFP-tagged DAF-16 in C. elegans

   Depositor

   Meng-Qiu Dong

   Article

   Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

   Insert

   DAF-16 (daf-16 Nematode)
   

   Tags

   GFP and HIS

   Expression

   Worm

   Available Since

   May 6, 2014

   Availability

   Academic Institutions and Nonprofits only

3. BRCA1 A10.2 gRNA

   Plasmid

   #90549

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BRCA1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   BRCA1 (Guide Designation A10.2)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #170810

   Purpose

   Lentiviral vector to co-express an spsgRNA with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEN_TT 3xFLAG-TWIST2

   Plasmid

   #197935

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged TWIST2

   Depositor

   Kevin Janes

   Article

   Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

   Insert

   TWIST2 (TWIST2 Human)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-CMV-eGFP

   Plasmid

   #193022

   Purpose

   AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the cytomegalovirus (CMV) promoter

   Depositor

   Bart Nieuwenhuis

   Article

   Nieuwenhuis et al Gene Ther. 2023 Jan 13. doi: 10.1038/s41

   Insert

   eGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLentiCas9-T2A-BFP

   Plasmid

   #78547

   Purpose

   Cas9 protein with BFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   T2A-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   In frame with Cas9

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMTKM07

   Plasmid

   #233479

   Purpose

   SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and URA selection.

   Depositor

   Vincent Martin

   Article

   Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TRE-KRAB-dCas9-IRES-BFP

   Plasmid

   #85449

   Purpose

   Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

   Depositor

   Eric Lander

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Insert

   KRAB-dCas9-IRES-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA Tag, and KRAB domain

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMBEC6

    Plasmid

    #187598

    Purpose

    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

    Insert

    nCas9-BEC-UGI; cas6f, gfp
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEF-XMAS-1xStop

    Plasmid

    #164411

    Purpose

    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-GFP

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BRCA2 A11.2 gRNA

    Plasmid

    #90550

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRCA2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BRCA2 (Guide Designation A11.2)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. BPK3082

    Plasmid

    #78742

    Purpose

    Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    LbCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-Parkin

    Plasmid

    #213547

    Purpose

    Transient mammalian expresssion of untagged Parkin

    Depositor

    Steven Altschuler, Lani Wu

    Article

    Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

    Insert

    Parkin (PRKN Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

    Plasmid

    #158972

    Purpose

    Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-VP64
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    pMecp2

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLJM1-Flag-Rheb

    Plasmid

    #165031

    Purpose

    expression of Flag tagged Rheb protein in mammalian cells

    Depositor

    Ken Inoki

    Article

    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

    Insert

    Rheb (RHEB Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pWN_U6-TRAC-miniGag

    Plasmid

    #228960

    Purpose

    miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMGS7 (AAVS1sgRNA)

    Plasmid

    #126582

    Purpose

    A CRISPR/Cas9 construct to target the AAVS1 locus in humans

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    AAVS1 sgRNA
    

    Expression

    Mammalian

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSCV-PIG-Cre

    Plasmid

    #50935

    Purpose

    retroviral expression of Cre recombinase

    Depositor

    Pier Pandolfi

    Article

    Chen et al Nature. 2005 Aug 4;436(7051):725-30.

    Insert

    Cre recombinase
    

    Use

    Cre/Lox and Retroviral

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDT-SuperFi-Cas9

    Plasmid

    #183630

    Purpose

    Expression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coli

    Depositor

    David Taylor

    Article

    Bravo et al Nature. 2022 Mar 2. pii: 10.1038/s41586-

    Insert

    SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …

    Promoter

    T7

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge