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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,912 results for: sta

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Showing: 5121 - 5140 of 14912 results

  1. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid
    #218710
    Purpose
    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    hSaCas9
    Chimeric guide for SaCas9
    Use
    AAV and CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218710-plasmid-map-sequence-id-433357

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  2. pRMC2-sec:msfGFP

    Plasmid
    #194914
    Purpose
    Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci
    Depositor
    Rikke Meyer
    Publication
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Expression
    Bacterial
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194914-plasmid-map-sequence-id-384625

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  9. pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

    Plasmid
    #156452
    Purpose
    Targeting vector to introduce an AID-eGFP cassette at the mouse RAD21 (SCC1) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA CCACGGTTCCATATTATCTG
    Depositor
    Elphege Nora
    Publication
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAD21, AID (Rad21 Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    none
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    156452-plasmid-map-sequence-id-310171

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  10. KCC2-pHext

    Plasmid
    #104076
    Purpose
    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)
    Depositor
    Igor Medyna
    Publication
    Friedel et al eNeuro. 2017 Jul 21;4(4). pii: ENEURO.00
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KCC2 (b isoform) (Slc12a5 Rat)
    Tags
    pHluorine tag inserted in the second putative tra…
    Expression
    Mammalian
    Mutation
    EcoR1 site in rat KCC2 was removed by silent muta…
    Promoter
    UbC
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104076-plasmid-map-sequence-id-199360

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  12. pCI-globin_del5UTR_PTC39-xrRNA-4H

    Plasmid
    #108367
    Purpose
    Expresses beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Publication
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
    Expression
    Mammalian
    Mutation
    PTC39
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108367-plasmid-map-sequence-id-209435

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  17. pTT3-ecdICAM1-ST3-His

    Plasmid
    #210666
    Purpose
    Expression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar)
    Depositor
    Omer Dushek
    Publication
    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.
    Insert
    Extracellular domain of human ICAM-1 fused to Spytag003 and Histag (ICAM1 Synthetic, Human)
    Tags
    Histag and Spytag003
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    210666-plasmid-map-sequence-id-417657

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Showing: 5121 - 5140 of 14912 results