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A1-LCD-20G+20S

Plasmid

#178683

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-20G+20S (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

twenty Gly residues replaced with Ser

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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SITS-MmCav1-APEX2

Plasmid

#170526

Purpose

Vector expression of MmCav1-APEX2 in Leishmania cell free system. Parton lab clone IYW

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Caveolin1 (Cav1 Mouse)

Use

Leishmania cell free expression clone

Tags

APEX2 and Lifeact

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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PMP34-hLOV-TEVcs-GAL4bd-VP64-V5

Plasmid

#170997

Purpose

HiLITR transcription factor with full-length PMP34 targeting sequence (Peroxisome)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

PMP34(FL)-NNES-hLOV-TEVcs(ENLYFQ-M)-GAL4bd-VP64-V5 (SLC25A17 Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

V5

Expression

Mammalian

Promoter

Ef1-alpha

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Pet22b-mEGFP-YAP-C-IDR

Plasmid

#166441

Purpose

Bacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP-C-IDR(266-504) (YAP1 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-neo-YAP-deltaCC

Plasmid

#166469

Purpose

Plasmid coding for YAP-deltaCC (delta298-359) and neomycin resistance in a lentiviral backbone.

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP-deltaCC, Neo (YAP1 Human)

Use

Lentiviral

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Myc-KLC1 delta Tail

Plasmid

#166965

Purpose

Expresses Myc-tagged KLC1 protein (1-495 aa) in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KLC1 (Klc1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

KLC1 (1-495aa)

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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RG6-BAP1_E11

Plasmid

#154025

Purpose

Bichromatic Fluorescent Splicing Reporter Minigene for Wild-Type BAP1 Exon 11

Depositor

Samuel Peña-Llopis

Article

Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

Insert

BAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)

Use

Splicing reporter

Tags

DsRed1, FLAG, SV40 NLS, and eGFP

Expression

Mammalian

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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RG6-BAP1_E7

Plasmid

#154023

Purpose

Bichromatic Fluorescent Splicing Reporter Minigene for Wild-Type BAP1 Exon 7

Depositor

Samuel Peña-Llopis

Article

Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

Insert

BAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)

Use

Splicing reporter

Tags

DsRed1, FLAG, SV40 NLS, and eGFP

Expression

Mammalian

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE (hygro) mEOS2 hYAP 1-1alpha

Plasmid

#124155

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

mEOS2-YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE (hygro)mEOS2 hYAP 1-1gamma

Plasmid

#124157

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

mEOS2-YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE (hygro) mEOS2 hYAP 1-1delta

Plasmid

#124158

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

mEOS2-YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE (hygro) mEOS2 hYAP 1-2alpha

Plasmid

#124159

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

mEOS2-YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE (hygro) mEOS2 hYAP 1-2beta

Plasmid

#124160

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

mEOS2-YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE (hygro) mEOS2 hYAP 1-2gamma

Plasmid

#124161

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

mEOS2-YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE (hygro) mEOS2 hYAP 1-1beta

Plasmid

#124156

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

mEOS2-YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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DOT1L-CC3

Plasmid

#111754

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

Contains coiled coil #3 (residues 613-661)

Promoter

T7

Available Since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DOT1L-CC1

Plasmid

#111756

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

contains coiled coil #1 (residues 506-555)

Promoter

T7

Available Since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DOT1L-CC2

Plasmid

#111755

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

Contains coiled coil #2 (residues 560-608)

Promoter

T7

Available Since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pAav-IQGAP1-PQS1-3xFLAG

Plasmid

#84885

Purpose

rAAV-based template for genome engineering of the IQGAP1 C-terminus containing PQS1 and 3xFLAG tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Inserts

IQGAP1 genomic region HR1 (IQGAP1 Human)
IQGAP1 genomic region HR2

Use

AAV

Tags

PQS1 3xFLAG

Promoter

no

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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DGKZ gRNA (BRDN0001147837)

Plasmid

#77464

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKZ

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKZ (DGKZ Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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