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We narrowed to 20,020 results for: cas9

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Showing: 5161 - 5180 of 20020 results

  1. pMA-SpCas9-g6

    Plasmid
    #80789
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80789-plasmid-map-sequence-id-161638

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  3. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139097
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dxCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139097-plasmid-map-sequence-id-273896

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  4. pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139095
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139095-plasmid-map-sequence-id-273892

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  5. pattB-LSL-AAVR-F2A-spCas9

    Plasmid
    #202459
    Purpose
    The plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.
    Depositor
    Jan Carette
    Publication
    Zengel et al Nat Methods. 2023 Jul;20(7):1070-1081. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVR (AU040320 Mouse)
    Use
    CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    mCherry fusion
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    202459-plasmid-map-sequence-id-402992

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  11. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid
    #218710
    Purpose
    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    hSaCas9
    Chimeric guide for SaCas9
    Use
    AAV and CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218710-plasmid-map-sequence-id-433357

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  12. GreenGate 2.0 Toolkit

    Plasmid kit
    #1000000241
    Purpose
    Compatible with GreenGate Cloning System (#1000000036). Includes modules for CRISPR/Cas9 genome editing, dexamethasone-inducible expression, and fluorescent plasma membrane and nuclear markers.
    Depositor
    Alexis Maizel
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector type
    Plant Expression
    Editing type
    CRISPR
    Cloning type
    Golden Gate (GreenGate)
    Available since
    April 11, 2024
    Availability
    Academic Institutions and Nonprofits only

  13. pMB1610_pRR-Puro

    Plasmid
    #65853
    Purpose
    Puromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9
    Depositor
    Marc Bühler
    Publication
    Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    split puromycin N-acetyltransferase coding sequence
    Use
    CRISPR and TALEN
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65853-plasmid-map-sequence-id-112015

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  14. pCMV-T7-SpCas9(MRKCRS)-P2A-EGFP (RAS2821)

    Plasmid
    #223102
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized nuclease SpCas9(MRKCRS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(MRKCRS)=D1135M, S1136R, G1218K, E1219C, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223102-plasmid-map-sequence-id-447669

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  15. pCMV-T7-SpCas9(LWRYEK)-P2A-EGFP (RAS2986)

    Plasmid
    #223094
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized nuclease SpCas9(LWRYEK)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(LWRYEK)=D1135L, S1136W, G1218R, E1219Y, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223094-plasmid-map-sequence-id-447661

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  16. pCMV-T7-SpCas9(MKRCMV)-P2A-EGFP (AHK162)

    Plasmid
    #223075
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized nuclease SpCas9(MKRCMV)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(MKRCMV)=D1135M, S1136K, G1218R, E1219C, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223075-plasmid-map-sequence-id-447667

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  17. pCMV-T7-SpCas9(MRRWMR)-P2A-EGFP (RAS2816)

    Plasmid
    #223068
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized nuclease SpCas9(MRRWMR)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223068-plasmid-map-sequence-id-447673

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  20. pR26

    Plasmid
    #127372
    Purpose
    Allows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.
    Depositor
    Lance Miller
    Publication
    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Mouse Targeting
    Promoter
    tight TRE promoter
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127372-plasmid-map-sequence-id-247988

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Showing: 5161 - 5180 of 20020 results