We narrowed to 14,090 results for: cas9

1. peft-3::cas-9::tbb-2 3'UTR

   Plasmid

   #48960

   Purpose

   this plasmid contains the codon optimized gene encoding Cas9 for C. elegans.

   Depositor

   Mario de Bono

   Article

   Chen et al Nucleic Acids Res. 2013 Sep 5.

   Insert

   cas9
   

   Use

   CRISPR

   Promoter

   eef-1A.1 (eft-3)

   Available Since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR1003

   Plasmid

   #71821

   Purpose

   Express Streptococcus pyogenes dCas9 (D10A, H840A)

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)
   

   Use

   CRISPR

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOsU3-esgRNA

   Plasmid

   #115629

   Purpose

   expression esgRNA in rice protoplasts

   Depositor

   Caixia Gao

   Article

   Li et al Genome Biol. 2018 May 29;19(1):59. doi:

   Insert

   OsU3p-esgRNA
   

   Use

   CRISPR

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. BCJJ339

   Plasmid

   #117501

   Purpose

   RPS5a:Cas9:E9 Golden Gate Level 1 Position 2

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:GCAA:RPS5a:Cas9-3(intron):E9:ACTA:BpiI
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtRPS5a

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJin-278

   Plasmid

   #125714

   Purpose

   ABA-inducible split T7 GFP vector using RNAPN d5-19

   Depositor

   Bryan Dickinson

   Article

   Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

   Insert

   PT7-GFP mRNA expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
   

   Expression

   Mammalian

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19, containing H2-Kb c57/BL6 WK9

   Plasmid

   #102641

   Purpose

   Encodes sequence of the murine H2-Kb allele

   Depositor

   Sai Reddy

   Article

   Kelton et al Sci Rep. 2017 Apr 4;7:45775. doi: 10.103

   Insert

   H2-Kd Balb/c
   

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNeurog2-Luciferase reporter

   Plasmid

   #64126

   Purpose

   Photoactivatable transcription system. Luciferase reporter under the control of the Neurog2 promoter.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   Neurog2 promoter
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Neurog2

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB8792

   Plasmid

   #126910

   Purpose

   Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   ADE2 gRNA
   

   Use

   Synthetic Biology

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AP582-2

   Plasmid

   #70049

   Purpose

   Expresses Ollas::mCherry::linker::H2B::V5 in bacteria

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

   Insert

   mCherry::linker::H2B
   

   Tags

   Ollas and V5

   Expression

   Bacterial

   Available Since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. PM-SP!TB

    Plasmid

    #48650

    Purpose

    Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Bacterial SP crRNA to prototspacer B
    

    Use

    CRISPR

    Promoter

    J23100 promoter

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCherry-U6-EMX1

    Plasmid

    #140581

    Purpose

    Expresses a gRNA and mCherry in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    gRNA EMX1
    

    Use

    CRISPR

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR1055

    Plasmid

    #71823

    Purpose

    Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLS

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Insert

    T7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
    

    Use

    CRISPR

    Available Since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ147

    Plasmid

    #69299

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 7 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJA50

    Plasmid

    #59931

    Purpose

    gRNA for cleavage at unc-58(e665) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    unc-58(e665) gRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. XX>sgRNA F+E

    Plasmid

    #87543

    Purpose

    Template 2 (of 2) for U6>sgRNA cassette OSO-PCR

    Depositor

    Lionel Christiaen

    Article

    Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

    Type

    Empty backbone

    Available Since

    Jan. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. BCJJ358

    Plasmid

    #117505

    Purpose

    RPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverse

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:tagt:RPS5a:Cas9_4:E9:ttgc:BpiI
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtRPS5a

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

    Plasmid

    #196710

    Purpose

    Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

    Depositor

    Bernhard Schmierer

    Article

    Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCBE3-HF

    Plasmid

    #133967

    Purpose

    Expresses HF-BE3 CRISPR base editor and gRNA scaffold in Streptomyces

    Depositor

    Yuhui Sun

    Article

    Zhong et al bioRxiv 630137

    Insert

    HF-BE3
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJA54

    Plasmid

    #60212

    Purpose

    cleavage near e1350 locus in sqt-1

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    sqt-1(e1350) gRNA1
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. AP588-1

    Plasmid

    #71278

    Purpose

    Expresses myc::TEV::mTagGFP::TEV::3Xflag in bacteria

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

    Insert

    tagGFP
    

    Tags

    TEV::3xFlag and myc::TEV

    Expression

    Bacterial

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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