We narrowed to 11,894 results for: pcas

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PET-SpCas9-HF1-plus-NLS-6xHis

Plasmid

#207381

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

SpCas9-HF1-plus

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, Q695A, Q926A, amino acids 1005-1013 replac…

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA

Plasmid

#108845

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

GAI

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA

Plasmid

#108843

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

GID1

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSD36_pCAG-PV1-FKBP-L1-TP901-327C-BGHpA

Plasmid

#108814

Purpose

Expresses aa327 to C-terminus of TP901 integrase fused to FKBP

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

TP901 integrase fragment aa327 to C-terminus

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Promoter

pCAG

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

Plasmid

#69227

Purpose

Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS4 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-Foldon-ATG9 (830-839)

Plasmid

#188091

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (830-839)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

Plasmid

#114090

Purpose

CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized MbCas12a

Tags

NLS(nucleoplasmin) and 3xHA

Expression

Mammalian

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBW1309_pCAG-PV1-FKBP-L1-CTD-ENTRY-BGHpA

Plasmid

#108837

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to FKBP tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

FKBP

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW1313_pCAG-PV1-PYL-L1-CTD-ENTRY-BGHpA

Plasmid

#108841

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to PYL tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

PYL

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2654_pCAG-PV1-pMag-L1-CTD-ENTRY-BGHpA

Plasmid

#108847

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to pMag tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

pMag

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)

Plasmid

#133788

Purpose

CAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized St3Cas9

Tags

NLS(SV40)-3xFLAG

Expression

Mammalian

Mutation

n/a

Promoter

CAG

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2

Plasmid

#69225

Purpose

Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS2 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-Foldon-ATG9 (692-839)

Plasmid

#188087

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (692-839)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2d10-364delta263-295-TEV-STREP

Plasmid

#171830

Purpose

Mammalian expression of human WIPI2d 10-364 delta263-295 with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep

Expression

Mammalian

Mutation

10-364delta263-295

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 S248A

Plasmid

#182047

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

S248A

Promoter

CAG

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 T258A

Plasmid

#182048

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

T258A

Promoter

CAG

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)

Plasmid

#133790

Purpose

CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized NmeCas9

Tags

NLS(SV40)-3xFLAG

Expression

Mammalian

Mutation

n/a

Promoter

CAG

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3

Plasmid

#69226

Purpose

Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS3 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

Plasmid

#113162

Purpose

A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

SpCas9

Tags

FLAG

Expression

Mammalian

Mutation

D10A

Promoter

CAG

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBW1311_pCAG-PV1-ABI-L1-CTD-ENTRY-BGHpA

Plasmid

#108839

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to ABI tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

ABI

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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