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pcDNA3/er-(n2)oxStayGold(c4)

Plasmid

#185822

Purpose

A soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.

Depositor

Atsushi Miyawaki

Article

Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

Insert

er-(n2)oxStayGold(c4)

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pPGK-SB13

Plasmid

#236078

Purpose

SB13 transposase, for use with Sleeping Beauty transponson plasmids

Depositor

Masashi Narita

Article

Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

Insert

SB13 transposase

Use

Sleeping beauty (sb) transposase plasmid

Promoter

PGK

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB402-omega

Plasmid

#74797

Purpose

Gateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

2xCaMV35S enhancer and omega

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-TS2126 RNA Ligase

Plasmid

#119941

Purpose

Express TS2126 RNA ligase in E. coli

Depositor

Takashi Ito

Article

Miura et al Nucleic Acids Res. 2019 May 22. pii: 549

Insert

TS2126 RNA ligase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-mAID-RPA194

Plasmid

#247355

Purpose

CRISPR/Cas9 donor plasmid to tag human RPA194 with mAID

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

RPA194 (POLR1A) (POLR1A Human)

Use

CRISPR

Tags

mAID

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGEM-HaloTag-RPA194

Plasmid

#247353

Purpose

CRISPR/Cas9 donor plasmid to tag human RPA194 with Halo tag

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

RPA194 (POLR1A) (POLR1A Human)

Use

CRISPR

Tags

HaloTag

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB405

Plasmid

#74799

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-MKLP1

Plasmid

#70145

Purpose

mammalian expression of MKLP1 with an eGFP fusion

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-αTAT1[2-236]

Plasmid

#27101

Depositor

Maxence Nachury

Article

Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

αTubulin K40 acetyltransferase (ATAT1 Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pX330-UBF

Plasmid

#247350

Purpose

Expresses SpCas9 and a sgRNA targeting the human UBF loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

UBF (UBTF Human)

Use

CRISPR

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pGEM-HaloTag-UBF

Plasmid

#247351

Purpose

CRISPR/Cas9 donor plasmid to tag human UBF with Halo tag

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

UBF (UBTF) (UBTF Human)

Use

CRISPR

Tags

HaloTag

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGEM-PAmCherry-RPA194

Plasmid

#247354

Purpose

CRISPR/Cas9 donor plasmid to tag human RPA194 with PAmCherry

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

RPA194 (POLR1A) (POLR1A Human)

Use

CRISPR

Tags

PAmCherry

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-MKLP1

Plasmid

#70154

Purpose

mammalian expression of MKLP1 with an mCherry fusion

Depositor

Masanori Mishima

Article

Joseph et al EMBO J. 2012 May 30;31(11):2604-14. doi:

Insert

MKLP1 (KIF23 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-BRCA1

Plasmid

#119007

Purpose

Mammalian expression of human BRCA1 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

BRCA1 (BRCA1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGK-HELLO LV

Plasmid

#202001

Purpose

Expresses HELLO neoantigen in tumor cells

Depositor

Nikhil Joshi

Article

Cui et al Cell. 2021 Dec 9;184(25):6101-6118.e13.

Insert

HELLO

Use

Lentiviral

Tags

FLAG

Promoter

pGK

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEF-BOS-RIG-I-Myc

Plasmid

#215872

Purpose

RIG-I-Myc expression vector for overexpression

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Genes (Basel). 2018 Oct 19;9(10):511. do

Insert

RIG-I (RIGI Human)

Expression

Mammalian

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr-AcGFP1-ORP9-PH

Plasmid

#214270

Purpose

Mammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

AcGFP1-ORP9(1-111) (Osbpl9 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHLSec2-FN-YPet

Plasmid

#65421

Purpose

Expresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7

Depositor

Harold Erickson

Article

Ohashi et al J Biol Chem. 2011 Nov 11;286(45):39188-9

Insert

human plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)

Tags

YPet

Expression

Mammalian

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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SP6-VEE-GFP

Plasmid

#58976

Purpose

expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

EGFP

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pBJ FLAG-hPar1

Plasmid

#53226

Purpose

mammalian expression of human Par1 with N-terminal FLAG tag

Depositor

Shaun Coughlin

Article

Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

Insert

Par1 (F2R Human)

Tags

FLAG and bovine prolactin signal sequence (MDSKGS…

Expression

Mammalian

Mutation

Deletion of aa1-39 (native signal peptide)

Promoter

SV40

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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