Addgene

We narrowed to 1,608 results for: nif

Showing: 1001 - 1040 of 1608 results

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pBA791

Plasmid

#186246

Purpose

dnap-S HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBA792

Plasmid

#186247

Purpose

dnap-F HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL:STS (DF)

Plasmid

#139791

Purpose

direct fusion of biosynthetic enzymes 4CL and STS

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

direct fusion of 4CL and STS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAW228

Plasmid

#113242

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader tAggtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

TTGGTAG->tAggtag

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW227

Plasmid

#113241

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Atggtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

TTGGTAG->Atggtag

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW207

Plasmid

#113247

Purpose

E. coli expression vector for IHFa K20A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa K20A (ihfA E. coli)
IHFb

Use

Tags

Expression

Bacterial

Mutation

K20A

Promoter

T7

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAW205

Plasmid

#113245

Purpose

E. coli expression vector for IHFa D14A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa D14A (ihfA E. coli)
IHFb

Use

Tags

Expression

Bacterial

Mutation

D14A

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAW217

Plasmid

#113239

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

GGTAG->ggtaC

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAW215

Plasmid

#113237

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

GGTAG->ggAag

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW214

Plasmid

#113236

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

GGTAG->gCtag

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW213

Plasmid

#113235

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Cgtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

GGTAG->Cgtag

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW211

Plasmid

#113233

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->AACGA

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

GGTAG->AACGA

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW210

Plasmid

#113189

Purpose

E. coli expression vector for untagged E. coli Cas1 H208A + Cas2 + CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

H208A

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW216

Plasmid

#113238

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtTg mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

GGTAG->ggtTg

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW203

Plasmid

#113243

Purpose

E. coli expression vector for IHFa E10A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa E10A (ihfA E. coli)
IHFb

Use

Tags

Expression

Bacterial

Mutation

E10A

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pGP4GY-cccdB

Plasmid

#83878

Purpose

Destination vector for EYFP fusions integrated at yeast Trp locus, expression from the GPD promoter

Depositor

Jennifer Nemhauser

Article

Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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human EB1-GFP mutagenized D88 K89 D94 K95 all converted to A (JB141)

Plasmid

#39302

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D88A, K89A, D94A and K95A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized Q109 W110 K112 K113 all converted to A (JB142)

Plasmid

#39303

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q109A, W110A, K112A and K113A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized K122 D123 Y124 D125 all converted to A (JB143)

Plasmid

#39304

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K122A, D123A, Y124A and D125A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized K148 K150 K151 all converted to A (JB144)

Plasmid

#39305

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K148A, K150A and K151A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized T249 D250 E251 all converted to A (JB146)

Plasmid

#39307

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T249A, D250A and E251A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pDAC565

Plasmid

#195242

Purpose

Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLBH559_Tet-HisCas14a1Locus

Plasmid

#112502

Purpose

Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pBA559

Plasmid

#186235

Purpose

pTet-LbuCas13a crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDAC439

Plasmid

#195237

Purpose

Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA560

Plasmid

#186236

Purpose

pTet-eLbuCas13a crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPP085

Plasmid

#158795

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti

Plasmid

#115795

Purpose

Expresses AcrIIA4 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrIIA4

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

EF-1a promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDAC435

Plasmid

#195238

Purpose

Mammalian expression of Csm complex from separate promoters; RFP backbone (Used for RNA knockdown)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1;FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; RFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBP-AsCas12a

Plasmid

#113430

Purpose

Expresses 10xHis-MBP fused to AsCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AsCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
JunB

Plasmid

#187903

Purpose

Expresses JunB in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

JunB (Junb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDAC627

Plasmid

#195240

Purpose

Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA620

Plasmid

#186240

Purpose

LbuCas13a Negative Control (RFP targeting)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

pTet-eLbuCas13a + crRNA negative control

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBP-FnCas12a

Plasmid

#113432

Purpose

Expresses 10xHis-MBP fused to FnCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

FnCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDAC569

Plasmid

#195241

Purpose

Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPAGFP-N1

Plasmid

#11909

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Has Service

Cloning Grade DNA

Insert

pPAGFP-N1

Use

Tags

Expression

Mammalian

Mutation

These plasmids are essentially the same as the Cl…

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pPAGFP-C1

Plasmid

#11910

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Has Service

Cloning Grade DNA

Insert

PAGFP

Use

Tags

Expression

Mammalian

Mutation

These plasmids are essentially the same as the Cl…

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pMJ806

Plasmid

#39312

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF525-EF1a-Hygro-P2A-mCherry-lenti

Plasmid

#115796

Purpose

Expresses mCherry in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

mCherry

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

EF-1a promoter

Available since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDAC446

Plasmid

#195239

Purpose

Mammalian expression of Csm complex from separate promoters; GFP backbone (Used for RNA knockdown)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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