Addgene

We narrowed to 18,394 results for: Tec;

Showing: 501 - 520 of 18394 results

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Ub-R-GFP

Plasmid

#11939

Depositor

Nico Dantuma

Article

Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

Insert

Ub-R-GFP

Use

Tags

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Arginine at…

Promoter

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-survivin

Plasmid

#196908

Purpose

visualization of midzone on anaphase and midbody later during mitosis in living mammalian cells

Depositor

Kenji Sugimoto

Article

Fukada et al Biotechniques. 2004 Oct;37(4):552, 554,

Insert

survivin

Use

Tags

EGFP

Expression

Mammalian

Mutation

E129K

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV mTurquoise2

Plasmid

#157777

Purpose

Expresses cyan fluorescent mTurquoise2 in cells

Depositor

Marc Tramier

Article

Bertolin et al ACS Sens. 2019 Aug 23;4(8):2018-2027. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pACYC-Skp-TF

Plasmid

#83921

Purpose

Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Trigger Factor

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pBlueKan+cysMPro

Plasmid

#187879

Purpose

Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.

Depositor

Gaylen Uhlich

Article

Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

CysM

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pPPAT-mCherry

Plasmid

#167162

Purpose

Expresses human PPAT-mCherry fusion in mammalian cells

Depositor

Stephen Benkovic

Article

Pedley et al Methods Mol Biol. 2018;1764:279-289. doi

Insert

PPAT (PPAT Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pEXPYR

Plasmid

#70030

Purpose

High yield protein secretion in Aspergillus nidulans

Depositor

Rolf Prade

Article

Segato et al Enzyme Microb Technol. 2012 Jul 15;51(2)

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

A. niger glucoamylase promoter (glaAp)4

Available since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMpGE010-gR082

Plasmid

#188553

Purpose

Binary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

gR082

Use

CRISPR; Entry vector

Tags

Expression

Mutation

Promoter

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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TtTMPV-Neo

Plasmid

#27993

Depositor

Scott Lowe

Article

Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

Insert

TtTMPV-Neo (shRen)

Use

RNAi and Retroviral; Fluorescent reporters

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

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p70rg 15

Plasmid

#17831

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pU1utr-EGFP

Plasmid

#140268

Purpose

U1A-dependent translational repression

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

U1A binding site

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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plet7d_stbC-EGFP

Plasmid

#140266

Purpose

LIN28-dependent translational repression

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

Stabilized hairpin of miR-let7d

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD*RFPEC2

Plasmid

#17823

Depositor

Jay Keasling

Article

Pfleger et al Biotechnol Bioeng. 2005 Dec 5. 92(5):553

Insert

E. coli optimized red flourescent protein (RFPec2)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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p70rg

Plasmid

#17827

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pET21-tVYN-Nt∆

Plasmid

#154431

Purpose

Plasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detection

Depositor

Ming Hammond

Article

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-NtYcgRΔ-NLucΔ4

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAptamerCassette-EGFP

Plasmid

#140288

Purpose

Backbone for making protein-responsive mRNA switches

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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BB1_O_34_AMA1_2.8

Plasmid

#90293

Purpose

BB1 with AMA1_2.8 sequence for transient plasmid propagation in A. niger between FS 34

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

AMA1_2.8

Use

Tags

Expression

Bacterial

Mutation

it is a truncated version of AMA1 (5.3 kb)

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST694_BB3_pTet-on_mttA_trpC_pcoxA:pyrGtrunc_AMA2.8

Plasmid

#90292

Purpose

BB3 for mttA integration with homologous flanks at pyrG locus, hygromycin resistance

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

mttA

Use

A niger

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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p70rg 1

Plasmid

#17828

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
p70rg 5

Plasmid

#17829

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only