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Plasmid#46267DepositorInsertVcl (VCL Chicken)
UseTagsEGFPExpressionMammalianMutationK1306A, R1308A, A50I mutation; talin binding defi…PromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-CHIKV-Structural polyprotein
Plasmid#215698PurposeProduces the Chikungunya virus structural polyprotein Capsid-E3-E2-6K-E1DepositorInsertCHIKV structural polyprotein Capsid-E3-E2-6K-E1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNAintron-CHIKV-Structural polyprotein with Nluc tagged E2
Plasmid#215699PurposeProduces the Chikungunya virus structural polyprotein Capsid-E3-NLucE2-6K-E1DepositorInsertCHIKV structural polyprotein Capsid-E3-NLucE2-6K-E1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Δ18 P.1
Plasmid#173476PurposeEncodes SARS-CoV-2 P.1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 Spike Truncated to remove 18 amino acids
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pattB-synaptobrevin-1-QFBDAD-G4MD-hsp70
Plasmid#46109DepositorInsertQFBDAD-G4MD
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized
Plasmid#184563PurposeCMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.DepositorInsertC16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)
UseLentiviralTagsExpressionMammalianMutationAll lysines were mutated to arginines. Codon-opti…PromoterCMVAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail
Plasmid#60392PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast, Synthetic)
UseTagsExpressionYeastMutationinternal 6xHis (see supplement figure 4), amino a…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJT9RFR
Plasmid#165067PurposeIntroducing GAL promoters-controlled sequiterpene nerolidol-producing module and Y-FAST and in S. cerevisiaeDepositorInsertT-RPL3>ERG20>P-GAL1-P-GAL2>Y-FAST>ErB1.2A-AcNES1>T-RPL41B
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSC-CMV-HA-Ast-3-NP-1-IRES-mCherry
Plasmid#159631Purposeexpresses HA Allatostatin-3, Neurophysin-1, IRES mCherry under the CSC promoterDepositorInsertAst (AstA Fly)
UseLentiviralTagsHA, IRES, mCherry, and neurophysin-1ExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1
Plasmid#213025PurposeExpresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cellsDepositorInsertLINE-1 ORF2p (1-1275)
UseTags3C-3xFlag (ORF2p)ExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT11F
Plasmid#165062PurposeIntroducing GAL promoters-controlled monterpene limonene-producing module and Y-FAST and in S. cerevisiaeDepositorInsertT-RPL3>ERG20-N127W>P-GAL1-P-GAL10>Y-FAST>T-ALD6-P-GAL2>ClLS>T-RPL41B
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394
Plasmid#128386PurposeExpresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruptionDepositorUseLentiviral, Luciferase, and Synthetic BiologyTagsC-terminal luc2 fragment and N-terminal luc2 frag…ExpressionMammalianMutationN terminal carbohydrate recognition domain fused …PromoterEF1A and IRESAvailable sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE
Plasmid#159630PurposeAAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoterDepositorInsertAst (AstA Fly)
UseAAVTagsHA, IRES, mCherry, and neurophysin-1ExpressionMammalianMutationPromoterDCA (same as CAG promoter)Available sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEQ276
Plasmid#83945Purposeexpress human cytomegalovirus IE1 and IE2 genes in eukaryotic cellsDepositorInsertIE1 and IE2
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUK421
Plasmid#44534DepositorInsertGAL1-HHF2
UseTagsExpressionYeastMutationPromoterGALAvailable sinceMay 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKR2.40
Plasmid#26952DepositorInsertpie-1promoter-GFP- gateway destination cassette B -pie-1 3’
UseTagsGFPExpressionWormMutationPromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
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AAV-hSyn-Ribo-jGCaMP8m (AAV1)
Viral Prep#167574-AAV1PurposeReady-to-use AAV1 particles produced from AAV-hSyn-Ribo-jGCaMP8m (#167574). In addition to the viral particles, you will also receive purified AAV-hSyn-Ribo-jGCaMP8m plasmid DNA. Synapsin-driven neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Ribo-jGCaMP8s (AAV1)
Viral Prep#167572-AAV1PurposeReady-to-use AAV1 particles produced from AAV-hSyn-Ribo-jGCaMP8s (#167572). In addition to the viral particles, you will also receive purified AAV-hSyn-Ribo-jGCaMP8s plasmid DNA. Synapsin-driven neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -