We narrowed to 2,320 results for: pcas9

1. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

   Plasmid

   #221550

   Purpose

   Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   gRNA targeting human TBK1 (TBK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   u6

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2T-cmv-SpCas9-TadDE-BlastR

   Plasmid

   #193850

   Purpose

   Express TadDE in mammalian cells with BlastR selection, Tol2 integration

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadDE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

   Plasmid

   #139994

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT3TS-spCas9-ABE-Umax-rest1

   Plasmid

   #222144

   Purpose

   Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2T-cmv-SpCas9-TadCBEa-BlastR

   Plasmid

   #193847

   Purpose

   Express TadCBEa in mammalian cells with BlastR selection, Tol2 integration

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadCBEa
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKRG3-mU6-PUb-3xFLAG-hSpCas9

   Plasmid

   #162161

   Purpose

   Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. CROPseq-EFS-SpCas9-P2A-blast

   Plasmid

   #106477

   Purpose

   single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens

   Depositor

   Alex Hewitt

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC1124 - pAAV MeCP2 SpCas9(D10A)

   Plasmid

   #112719

   Purpose

   An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   SpCas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Mecp2

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pspCas9-3'UTR-ST2-com-vector

   Plasmid

   #136269

   Purpose

   Expresses SpCas9 and sgRNA Scaffold

   Depositor

   Baisong Lu

   Article

   Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

   Insert

   SpCas9 and sgRNA scaffold
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pspCas9-3'UTR-Tetra-com-vector

    Plasmid

    #132553

    Purpose

    Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Inserts

    Cas9
    sgRNA scaffold with com replacing the Tetraloop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9

    Plasmid

    #107857

    Purpose

    To knock-out TDP43

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    gRNA against TARDBP (Exon 1) (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11

    Plasmid

    #161923

    Purpose

    To generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    sgRNA targeting HSD17B11 exon 1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. PET-Sniper SpCas9-NLS-6xHis

    Plasmid

    #207374

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Sniper SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F539S, M763I, K890N

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSD_N-SpCas9-1-573-FRB-1xNLS

    Plasmid

    #107298

    Purpose

    Expresses N terminal SpCas9 (1-573 aa) fused to FRB in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    Truncated SpCas9 (aa 1-573) fused to FRB
    

    Use

    CRISPR

    Tags

    FRB and SV40 NLS

    Expression

    Mammalian

    Mutation

    Truncated SpCas9 (aa 1-573)

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid

    #174140

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-FLAG-SpCas9-HF1-plus

    Plasmid

    #126775

    Purpose

    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A; Q695A; Q926A; amino acids 1005-1013 replac…

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pspCas9-POLI-ST2-com vector

    Plasmid

    #176234

    Purpose

    Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-dSpCas9-HF1-VP64-6xHis

    Plasmid

    #92118

    Purpose

    Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, Q926A

    Promoter

    T7

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMC-Z-SpCas9-gRNA-repeat-polyT-11

    Plasmid

    #197781

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    gRNA repeat for U6 sgRNA assembly
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-Flag-eSpCas9 (without sgRNA)

    Plasmid

    #92354

    Purpose

    Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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