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pMX1418

Plasmid

#127856

Purpose

Expresses EGFP-IPNN-MCAK in mammalian cells

Depositor

Linda Wordeman

Article

Domnitz et al J Cell Biol. 2012 Apr 16;197(2):231-7. d

Insert

IPNN-MCAK

Tags

EGFP

Expression

Mammalian

Mutation

mutation is I97N and P98N

Promoter

CMV

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1a-mRor2deltaPro

Plasmid

#22634

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 delta Pro (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of Pro domain

Available Since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only
UAS-Syt::3xHalo7

Plasmid

#87649

Purpose

For creating drosophila transgenics expressing UAS-Syt::3xHalo7

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Syt::3xHalo7

Expression

Insect

Mutation

S265G in Syt (please see depositor's commen…

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD009

Plasmid

#116850

Purpose

Expresses araC constitutively and alpha-hemolysin under a pBAD promoter; alternate name pSB1A2-AD009

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
alpha-HL

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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Neg. Ctrl_sgRNA

Plasmid

#111298

Purpose

CRISPR KO murine neg. ctrl.

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

NegCtrl sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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KFS2MT

Plasmid

#44624

Depositor

Robert Oshima

Article

Cecena et al Am J Pathol. 2006 Jan;168(1):310-20.

Insert

keratin 18 gene (KRT18 Human)

Use

Mouse Targeting

Tags

polyomavirus middle T antigen (PyMT)

Available Since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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MsCLYBL_sgRNA1

Plasmid

#111294

Purpose

CRISPR KO murine CLYBL

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA1 against murine CLYBL

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRC01

Plasmid

#114680

Purpose

sfGFP.Strands 1-9

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

sfGFP Strands 1-9

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMR-A-4

Plasmid

#138518

Purpose

P3b

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBT138b-proA

Plasmid

#138514

Purpose

P3g

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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HOXD10-pSG5

Plasmid

#21008

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

HOXD10 (HOXD10 Human)

Expression

Mammalian

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
CLAPon

Plasmid

#213405

Purpose

Peroxidase (APEX) reporter for TEV protease cleavage

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

E5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA

Plasmid

#64983

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pC0.286

Plasmid

#119627

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

AbRChl

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAG373-mito-redFAST

Plasmid

#160358

Purpose

Expresses mito-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

mito-redFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBSKΔB

Plasmid

#177268

Purpose

Backbone vector for STREAMING-tag knockin vector

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-FKBP-TEVp

Plasmid

#178867

Purpose

Mammalian expression of mCherry-FKBP-TEVprotease(Δ220-242)

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

mCherry-FKBP-TEVprotease(Δ220-242)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pRK1036

Plasmid

#17728

Depositor

David Waugh

Article

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

NusG (nusG Aquifex aeolicus)

Tags

His6 and MBP

Expression

Bacterial

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB

Plasmid

#178862

Purpose

Mammalian expression of PB1-AzamiGreen-FRB

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEBB-XIAP1-351

Plasmid

#11560

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAP1-351 (XIAP Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 352-496

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only