We narrowed to 32,520 results for: REP

1. pET15b MOSPD2 RD/LD [282-490]

   Plasmid

   #186470

   Purpose

   Expression of human MOSPD2 (MSP domain from residue 282 to 490; mutant R404D/L406D, unable to bind FFAT motifs), fused to a 6HIS tag, in bacteria

   Depositor

   Catherine Tomasetto

   Article

   Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   MSP domain [282-490]

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b MOSPD2 [282-490]

   Plasmid

   #186469

   Purpose

   Expression of human MOSPD2 (MSP domain from residue 282 to 490), fused to a 6HIS tag, in bacteria

   Depositor

   Catherine Tomasetto

   Article

   Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   MSP domain [282-490]

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT22gCfpUbiG4FER4P2A(#249)

   Plasmid

   #184070

   Purpose

   cyclofen-inducible GAL4 activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

   Insert

   Gal4bdFF-ERT2-P2A
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   Expression

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Slc1a3-CreERT2 Targeting Vector

   Plasmid

   #129409

   Purpose

   Gene targeting vector to knock-in 2A-CreERT2 into the mouse Slc1a3 locus in place of a STOP codon

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

   Insert

   Slc1a3-2A-CreERT2 Targeting Vector (Slc1a3 Mouse)
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. px335 Slc1a3 nickase 1

   Plasmid

   #129410

   Purpose

   Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

   Insert

   sgRNA directing Cas9n to the proximity of Slc1a3 STOP codon (Slc1a3 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. px335 Slc1a3 nickase 2

   Plasmid

   #129411

   Purpose

   Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

   Insert

   sgRNA targeting Slc1a3 (Slc1a3 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-Hygro-EF1A-RG-Linker-eGFP

   Plasmid

   #176071

   Purpose

   EGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   RG
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCRISPRv2-POLB-KO-g1

   Plasmid

   #176090

   Purpose

   Cas9 plus POLB gRNA #1; contains a puromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   POLB sgRNA #1 (POLB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-Hygro-EF1A-KR-Linker-eGFP

   Plasmid

   #176066

   Purpose

   EGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   KR
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA4 3xFLAG Halo G-Linker nsp1

    Plasmid

    #175426

    Purpose

    Expresses G-linker CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    G-linker nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    G-linker in place of the RNase destabilization do…

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA4 3xFLAG Halo R119A K120A nsp1

    Plasmid

    #175428

    Purpose

    Expresses R119A K120A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 R119A K120A
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminal mutants R119A K120A

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA4 3xFLAG Halo E55A E57A K58A nsp1

    Plasmid

    #175427

    Purpose

    Expresses E55A E57A K58A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 E55A E57A K58A
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminal mutants E55A E57A K58A

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA4 3xFLAG Halo E36A E37A nsp1

    Plasmid

    #175429

    Purpose

    Expresses E36A E37A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 E36A E37A
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminal mutants E36A E37A

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Bmpr2ΔEC gRNA resistant

    Plasmid

    #163413

    Purpose

    Expresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The extracellular domain (27-150 aa) is deleted. …

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-Bmpr2ΔTail gRNA resistant

    Plasmid

    #163412

    Purpose

    Expresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The C-terminal tail domain (530-1038 aa) is delet…

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQlinkG2-PTPIP51(236-470)

    Plasmid

    #170534

    Purpose

    Expresses Cleavable GST-tagged PTPIP51 TPR domain (236-470) in E. Coli

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFASTbac-HisGST-PTPIP51(36-470)

    Plasmid

    #170531

    Purpose

    Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 )
    

    Use

    Tags

    GST tag and His tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQlinkH-PTPIP51(236-470)

    Plasmid

    #170530

    Purpose

    Expresses Cleavable His-tagged PTPIP51 TPR domain (236-470) in E. Coli

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (236-470) (RMDN3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. YF

    Plasmid

    #171459

    Purpose

    Expresses YF, a FiCE of iCMM, in mammalian cells

    Depositor

    Takafumi Miyamoto

    Article

    Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

    Insert

    EYFP-FKBP12
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. mYF

    Plasmid

    #171460

    Purpose

    Expresses mYF, a CiCE of NiCMM, in mammalian cells

    Depositor

    Takafumi Miyamoto

    Article

    Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

    Insert

    EYFP(A207K)-FKBP12
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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