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pMBI

Plasmid

#17816

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pMCV-R17a

Plasmid

#24729

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

Full genome of Human Merkel Cell polyomavirus

Use

Cloning vector

Available Since

May 19, 2010

Availability

Academic Institutions and Nonprofits only

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pLU-YFP-ICP0

Plasmid

#134563

Purpose

Expresses YFP-ICP0 fusion protein

Depositor

Susan Janicki

Article

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Insert

YFP-ICP0

Tags

YFP

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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NM R2E2 GFP

Plasmid

#1208

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 NM domain (SUP35 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

2 additional copies of second oligopeptide repeat…

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pU6-Lb-crRNA

Plasmid

#78957

Purpose

Cloning vector for expression of LbCpf1 crRNA. It contains BsmB1 site for cloning.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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vHB16

Plasmid

#193455

Purpose

Expression of GFP-Cas9-NLS and gRNA in A. castellanii

Depositor

Chantal Abergel

Article

Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

Insert

tRNA-gRNA

Use

CRISPR

Promoter

U6

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pESC-HIS (V5 and HA)

Plasmid

#222487

Purpose

Co-expression of two genes in yeast with V5 and HA tags and HIS3 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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6972 pPROEX-HTb-SSA1

Plasmid

#1231

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Ssa1 (SSA1 Budding Yeast)

Tags

his tag (tev cleavable)

Expression

Bacterial

Available Since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only
pLRD11

Plasmid

#208263

Purpose

Production of sclareol in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
abCAS
ssSS

Expression

Bacterial

Mutation

abCAS(D537A)

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUM

Plasmid

#64176

Purpose

harbours marker genes URA3 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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6798 pJC25NMstop

Plasmid

#1228

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

NM (SUP35 Budding Yeast)

Expression

Bacterial

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pCCL-GKL1ShortMutPRC-Ug1Lg2starTGA

Plasmid

#52347

Purpose

Integration cassette to create p1-Short-UL*(TGA)

Depositor

Chang Liu

Article

Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

Insert

leu*

Use

P1 integration vector

Expression

Bacterial

Mutation

changed S2F, A3V, Q180*

Promoter

UCS from pGKL2 ORF5

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLO87

Plasmid

#91726

Purpose

mycobacterial expression of mCherry from Hsp60 promoter

Depositor

Graham Hatfull

Article

Oldfield et al J Bacteriol. 2014 Oct;196(20):3589-97. d

Insert

hsp60 promoter

Use

Mycobacterial expression

Tags

mCherry

Promoter

hsp60

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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p7SKU6SBpur

Plasmid

#160329

Purpose

dual sh RNA vector expressing cfSBFP2 and a puromycin selection marker

Depositor

Ikuo Wada

Article

Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

Type

Empty backbone

Tags

cfSBFP2

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1_sgRNA

Plasmid

#100554

Purpose

Expresses AAVS1 sgRNA. Target sequence: GGGGCCACTAGGGACAGGAT

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

AAVS1 sgRNA

Promoter

U6

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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CytoCKAR

Plasmid

#14870

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Tags

CFP, NES, and YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pJW1511

Plasmid

#62365

Purpose

pEF1a-puro-T2A-ATHmmuRPL10A

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

Puro-T2A-AVITEVHAmmuRPL10A

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pEiLOV-N1

Plasmid

#26588

Depositor

John Christie

Article

Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

Insert

iLOV

Expression

Mammalian

Available Since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only

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RPO41

Plasmid

#205072

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Tang et al J Biol Chem. 2009 Feb 27;284(9):5514-22.

Insert

RPO41 (RPO41 Budding Yeast)

Expression

Bacterial

Mutation

Lacks N Terminal 100 amino acids

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pJOE4847.2

Plasmid

#135086

Purpose

rhamnose-inducible expression vector

Depositor

Josef Altenbuchner

Article

Motejadded et al Biotechnol Lett. 2009 Apr;31(4):543-9. d

Insert

MalE-6xHis-Ulp1

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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