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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,582 results for: eng

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Showing: 5181 - 5200 of 14582 results

  1. pCAG-AaCas12b(R911A)-2AeGFP

    Plasmid
    #121961
    Purpose
    Mammalian expression, Transcription regulation, deactivated AaCas12b
    Depositor
    Wei Li
    Publication
    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Catalytically deactivated AaCas12b
    Tags
    3xHA tag
    Expression
    Mammalian
    Mutation
    R911A
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    121961-plasmid-map-sequence-id-232188

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  8. CD4_LD 0_CS G_tTA-BFP

    Plasmid
    #58869
    Purpose
    MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Publication
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    58869-plasmid-map-sequence-id-94230

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  14. pBK-Ma pylRS nitroY/haloY-F5

    Plasmid
    #212125
    Purpose
    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.
    Depositor
    Ryan Mehl
    Insert
    M. alvus PylRS 3-NY/HaloY F5
    Expression
    Bacterial
    Mutation
    CTG125CTT, N166A V168S, A223C, W239R
    Promoter
    GlnS (constitutive)
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    212125-plasmid-map-sequence-id-443626

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  17. pDIV303

    Plasmid
    #204978
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and amdS selection marker
    Depositor
    Uffe Mortensen
    Publication
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    amdS
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    two silent mutations (nt177T>C, nt879C>T)
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    204978-plasmid-map-sequence-id-415579

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  18. pNMSB84

    Plasmid
    #199309
    Purpose
    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605
    Depositor
    Michael Krieg
    Publication
    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4
    Insert
    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
    Expression
    Worm
    Mutation
    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…
    Promoter
    flp-18
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    199309-plasmid-map-sequence-id-404774

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Showing: 5181 - 5200 of 14582 results