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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 5201 - 5220 of 7910 results

  1. TFORF0081

    Plasmid
    #141509
    Purpose
    Lentiviral vector for overexpressing the KCNIP2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KCNIP2 (KCNIP2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141509-plasmid-map-sequence-id-276663

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  2. psiCHECK-VIM270-gSM

    Plasmid
    #215870
    Purpose
    This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.
    Depositor
    Kumiko Ui-Tei
    Article
    Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d
    Insert
    3 tandem repeats of target site (complementary to seed seq of guide strand of siRNA for VIM) is inserted to 3'UTR of RLuc (VIM Human)
    Expression
    Mammalian
    Available Since
    May 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    215870-plasmid-map-sequence-id-431352

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  3. psiCHECK-VIM270-gCM

    Plasmid
    #215868
    Purpose
    This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.
    Depositor
    Kumiko Ui-Tei
    Article
    Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d
    Insert
    target site which is completely complementary to guide strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
    Expression
    Mammalian
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215868-plasmid-map-sequence-id-431664

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  12. GPR160-Tango

    Plasmid
    #66333
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR160 (GPR160 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. TFORF2756

    Plasmid
    #144156
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BARHL2 (BARHL2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144156-plasmid-map-sequence-id-392335

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Showing: 5201 - 5220 of 7910 results