We narrowed to 58,587 results for: plasmid
-
Plasmid#204734PurposeImproved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.InsertOptimized neurogenin-2
ExpressionMammalianAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
PE7_AAVS1_knockin_HDR_donor
Plasmid#223001PurposeHDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPE7
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianPromoterEF1αAvailable SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CH
Plasmid#26117PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneTagsHisExpressionBacterialPromoterT7-lacOAvailable SinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-Archon1-EGFP
Plasmid#108417PurposeAAV production plasmid encoding for Archon1 fluorescent voltage reporterDepositorInsertArchon1-EGFP
UseAAVExpressionMammalianPromoterCaMKIIAvailable SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
HK2
Plasmid#25529PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceSept. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
EHMT2
Plasmid#25503PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
KRASB
Plasmid#25153PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 6, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDH-SFFV-GLuc-CreERT2-mCherry
Plasmid#178185Purposethe plasmid promotes the constitutive expression of CreERT2 to enable intracellular recombination, Gaussia Luc can be used for in vivo imaging and mCherry is used as a marker for ex vivo analyses.DepositorInsertsGLuc
CreERT2
mCherry
UseLentiviralAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWN_U6-B2M-miniGag-Cas9
Plasmid#228958PurposeminiEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)DepositorInsertminiGag-Cas9
UseCRISPRExpressionMammalianAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM4D(Gi)-mCherry (AAV1)
Viral Prep#50477-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CaMKIIa-hM4D(Gi)-mCherry (#50477). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM4D(Gi)-mCherry plasmid DNA. CaMKIIa-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable SinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (AAV5)
Viral Prep#198511-AAV5PurposeReady-to-use AAV5 particles produced from pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (#198511). In addition to the viral particles, you will also receive purified pAAV_hSyn-DIO-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven Cre-dependent expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmScarlet (Cre-dependent)Available SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRL056 pDESTattB_exorh:EGFP
Plasmid#213510PurposeattB-containing pDEST plasmid, for use in Multisite Gateway reactionDepositorTypeEmpty backboneUseZebrafish expressionAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
PermaPhos Kit
Plasmid Kit#1000000226PurposePlasmids to express proteins with site-specific, non-hydrolyzable phosphoserine (nhpSer) residues in E. coli.DepositorApplicationGene Expression and LabelingVector TypeBacterial ExpressionAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 (AAV9)
Viral Prep#172922-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 (#172922). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 plasmid DNA. hSyn-driven expression of jGCaMP8m in axons with cytosolic expression of mRuby3. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-BrCas12b-RFND
Plasmid#195339PurposeExpresses Engineered BrCas12b in BacteriaDepositorInsertBrCas12b
UseCRISPR and Synthetic BiologyTags6xHis and 6xHis, MBP, TEV cleavage siteExpressionBacterialMutationR160E, F208W, N524V, D868VPromoterT7Available SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas12Max-ABE
Plasmid#195335Purposevector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized dCas12Max-ABE
ExpressionMammalianMutationN243R, E336R, D1049APromoterCBh, CMV, hU6Available SinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-lck-jGCaMP8s (AAV9)
Viral Prep#176761-AAV9PurposeReady-to-use AAV9 particles produced from pZac2.1-GfaABC1D-lck-jGCaMP8s (#176761). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-lck-jGCaMP8s plasmid DNA. GfaABC1D-driven expression of membrane-bound calcium sensor GCaMP8s. These AAV preparations are suitable purity for injection into animals.DepositorPromoterGfaABC1DAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-off TauRD (P301L/V337M)
Plasmid#188572PurposeDox-repressible expression of the Tau repeat domainDepositorInsertTau repeat domain (MAPT Human)
UseLentiviralTagsMycExpressionMammalianMutationP301L and V337MPromoterpTight TREAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
CVB3 IRES
Plasmid#189968PurposePart 2 plasmid to introduce the CVB3 IRES into circRNAsDepositorInsertCVB3 IRES
UseCloningPromoterN/AAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-SpRY-NLS-6xHis (gbr2101)
Plasmid#181743Purposebacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tagDepositorInsertbacterial codon optimized SpRY
UseIn vitro transcription; t7 promoterTagsBPNLS(SV40)-6xHis and MKIEE MBP tagExpressionBacterialMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterT7Available SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only