We narrowed to 16,828 results for: otos

1. pAAV-EF1a-DIO-ASAP3Kv-WPRE

   Plasmid

   #132330

   Purpose

   expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

   Depositor

   Michael Lin

   Article

   Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

   Insert

   ASAP3KV
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCMVblast_SLC38A2_wt

   Plasmid

   #156180

   Purpose

   CMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.

   Depositor

   Alec Kimmelman

   Article

   Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

   Insert

   SLC38A2 (SLC38A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Silent mutations to prevent targeting by sgRNA se…

   Promoter

   CMV

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HDM-SARS2-Spike-delta21

   Plasmid

   #155130

   Purpose

   Encodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotyping

   Depositor

   Jesse Bloom

   Article

   Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

   Insert

   SARS-CoV-2 Spike-delta21 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized to H. sapiens using IDT codon opt…

   Promoter

   CMV

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSIREN-RetroQ-BPTF-sh5

   Plasmid

   #83045

   Purpose

   MMLV LTR plasmid pSIREN-RetroQ-BPTF-sh5

   Depositor

   Joe Landry

   Article

   Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

   Insert

   shRNA #5 to BPTF (BPTF, Bptf Mouse, Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAAV-hSyn-JEDI-2P-WPRE

   Plasmid

   #179464

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Has Service

   AAV9

   Insert

   JEDI-2P
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-MYO5C

   Plasmid

   #135404

   Purpose

   Expresses GFP-tagged human MYO5C in mammalian cells.

   Depositor

   Richard Cheney

   Article

   Jacobs et al Mol Biol Cell. 2009 Nov;20(21):4471-88.

   Insert

   MYO5C (MYO5C Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Relative to the GenBank MYO5C cDNA sequence repor…

   Promoter

   CMV

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1α1.1-ChrimsonR-GFP

   Plasmid

   #122063

   Purpose

   AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   EF1a（1.1kb short version)

   Available since

   March 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICE-EGFP-FLAG-PSMD2

   Plasmid

   #161917

   Purpose

   Expresses human PSMD2 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   26S proteasome non-ATPase regulatory subunit 2 (PSMD2 Human)
   

   Use

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-Syn-FLEX-rc [Chronos-tdTomato]

   Plasmid

   #84481

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin promoter

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CaMKIIa-JEDI-2P-Kv-WPRE

    Plasmid

    #179465

    Purpose

    Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Insert

    JEDI-2P-Kv
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-JEDI-2P-Kv-WPRE

    Plasmid

    #179463

    Purpose

    Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Has Service

    AAV9

    Insert

    JEDI-2P-Kv
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-Syn-Chronos-tdTomato

    Plasmid

    #62726

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the Syn promoter. Using bGHpA signal. tdTomato has codons varied between the first and second tandem repeats to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin promoter

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Epac-S-H189

    Plasmid

    #170350

    Purpose

    mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

    Insert

    mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMSCVpuro-Mito-DsRed1

    Plasmid

    #87379

    Purpose

    Retroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).

    Depositor

    Björn Stork

    Article

    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

    Insert

    TXN2 mitochondrial transit peptide (TXN2 Human)
    

    Use

    Retroviral

    Tags

    DsRed1

    Expression

    Mammalian

    Mutation

    First 60 aa only

    Promoter

    Retroviral 5' LTR promoter

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-CAG-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid

    #130909

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV8

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato (codon diversified version)

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    CAG

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CaMKII-ChrimsonR-tdTomato

    Plasmid

    #99231

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    CaMKII

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQCXIP-mCherry-Halo-YAP1

    Plasmid

    #128336

    Purpose

    Retrovirus construct to express mCherry-Halo-YAP1

    Depositor

    Yutaka Hata

    Article

    Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry and Halo-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CAG-FLEX-rc [Chronos-tdTomato]

    Plasmid

    #84484

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the CAG promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-Syn-FLEX-rc[Chronos-GFP]

    Plasmid

    #62722

    Purpose

    AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin promoter

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-Syn-FLEX-rc [ChrimsonR-GFP]

    Plasmid

    #84480

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    human synapsin promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-Syn-FLEX-rc[CoChR-GFP]

    Plasmid

    #62724

    Purpose

    AAV-mediated expression of CoChR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    CoChR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin promoter

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-mTubb3

    Plasmid

    #87116

    Purpose

    AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGL3-mFgf21pro2-luc

    Plasmid

    #101798

    Purpose

    Reporter for mouse Fgf21 promoter activity

    Depositor

    Seiichi Oyadomari

    Article

    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

    Insert

    Fibroblast growth factor 21 promoter (Fgf21 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGL3-mFgf21pro3-luc

    Plasmid

    #101799

    Purpose

    Reporter for mouse Fgf21 promoter activity

    Depositor

    Seiichi Oyadomari

    Article

    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

    Insert

    Fibroblast growth factor 21 promoter (Fgf21 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT7NT*-Bri2 113-231 R221E

    Plasmid

    #138134

    Purpose

    Expresseds human Bri2 BRICHOS R221E mutant in E. coli

    Depositor

    Jan Johansson

    Article

    Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 1

    Insert

    human Bri2 BRICHOS R221E (ITM2B Human)
    

    Use

    Tags

    NT* tag derived from spider silk proteins

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV.CAG-FLEX.iAChSnFR-Venus

    Plasmid

    #137959

    Purpose

    Expresses FLEXed iAChSnFR (yellow version) under CAG promoter

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Insert

    iAChSnFR (yellow variant)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV.CAG.iAChSnFR-NULL

    Plasmid

    #137956

    Purpose

    Expresses iAChSnFR (non-binding variant) under CAG promoter

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Insert

    iAChSnFR (non-binding variant)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Y140A binding site mutant

    Promoter

    CAG

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-1BPNLS-Cas9-1BPNLS

    Plasmid

    #87108

    Purpose

    BPNLS-Cas9-BPNLS expression plasmid

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    1BPNLS-Cas9-1BPNLS
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-GFAP-JEDI-2P-WPRE

    Plasmid

    #179469

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for astrocytes specific expression using the promoter GFAP

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Insert

    JEDI-2P
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSBbi-moxGFP-P2A-T2A-Puro

    Plasmid

    #176893

    Purpose

    Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP and two additional genes of interest

    Depositor

    Jorge Luis Arias-Arias

    Article

    Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

    Insert

    moxGFP-P2A-T2A
    

    Use

    Transposon

    Tags

    moxGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge