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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 24,796 results for: CHI;

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Showing: 5241 - 5260 of 24796 results

  5. pEVOL-ABK

    Plasmid
    #126035
    Purpose
    Efficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Nat Commun. 2019 Sep 6;10(1):4046. doi:
    Inserts
    tRNA synthetase 1
    tRNA synthetase 2
    tRNA for TAG codon
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    araBAD promoter, glnS promoter, and proK promoter
    Available Since
    Sept. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    126035-plasmid-map-sequence-id-244699

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  8. pDule-Tet2.0

    Plasmid
    #85496
    Purpose
    Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044
    Insert
    Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32G L65Q F108S Q109D D158S L162N
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85496-plasmid-map-sequence-id-169865

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  10. pBpaRS3tRNA

    Plasmid
    #197102
    Purpose
    To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d
    Insert
    TyrRS variant, amber suppressor tRNA
    Expression
    Bacterial
    Mutation
    Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)
    Available Since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    197102-plasmid-map-sequence-id-388022

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  12. pEVA1

    Plasmid
    #58953
    Purpose
    Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.
    Depositor
    Thomas Fox, Thomas Mason
    Article
    Sanchirico et al EMBO J. 1998 Oct 1;17(19):5796-804.
    Insert
    VAR1u, VAR1 recoded for expression from the nucleus
    Use
    Yeast ecoli shuttle vector
    Tags
    preCox4 mitochondrial matrix targeting signal
    Expression
    Yeast
    Mutation
    Synthetic gene in standard genetic code
    Promoter
    ADH1
    Available Since
    Aug. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 5241 - 5260 of 24796 results