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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 126,203 results for: CIL

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Showing: 5241 - 5260 of 126203 results

  3. RED

    Plasmid
    #139835
    Purpose
    Expresses RNase H1-EGFP- dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Article
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Expression
    Mammalian
    Mutation
    dCas9 protein contains two mutations: D10A and H8…
    Promoter
    tet-inducible CMV
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139835-plasmid-map-sequence-id-310615

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  6. pT7TS

    Plasmid
    #17091
    Depositor
    Paul Krieg
    Type
    Empty backbone
    Available Since
    June 5, 2008
    Availability
    Academic Institutions and Nonprofits only
    17091-plasmid-map-sequence-id-440478

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  8. pDG462

    Plasmid
    #100903
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100903-plasmid-map-sequence-id-196607

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  9. pLenti-Synapsin-pHoenix-WPRE

    Plasmid
    #70111
    Purpose
    lentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesicles
    Depositor
    Christian Rosenmund
    Article
    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n
    Insert
    pHoenix (Syp Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)
    Use
    Lentiviral
    Tags
    Arch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…
    Promoter
    Synapsin
    Available Since
    Nov. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    70111-plasmid-map-sequence-id-177119

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  15. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

    Plasmid
    #113865
    Purpose
    Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
    Depositor
    Manfred Ogris
    Article
    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d
    Inserts
    CMV-eGFP
    TOP-iRFP
    PTCH1-mTurquoise2
    CBF-tdTomato
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    113865-plasmid-map-sequence-id-220242

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Showing: 5241 - 5260 of 126203 results