We narrowed to 160,365 results for: DES

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ABE8e-dimer

Plasmid

#138490

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-nSpCas9

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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ECFP-IKB

Plasmid

#111193

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

IKB-alpha

Tags

ECFP

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-CRY2-OCRL

Plasmid

#79565

Purpose

expression of GFP-tagged CRY2PHR fused to inositol 5-phosphatase domain of OCRL

Depositor

Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

OCRL (OCRL Human)

Tags

CRY2 (photolyase homology region domain of Arabid…

Expression

Mammalian

Mutation

aa 234_539

Promoter

CMV

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDS2142

Plasmid

#184273

Purpose

Tet-off genome insertion system

Depositor

Dominique Sanglard

Article

Lemberg et al PLoS Pathog. 2022 Apr 11;18(4):e1010012.

Insert

CatTA

Expression

Yeast

Promoter

TDH3

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2)2(S4)2-gs

Plasmid

#228072

Purpose

Mammalian expression of coiled coil segments (S2)x2-(S4)x2, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2, S4

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCDHblast-flx-sORF-Katushka

Plasmid

#179457

Purpose

Lentiviral vector encoding a CRE inducible Katushka gene

Depositor

Ton Schumacher

Article

Bresser et al Nat Immunol. 2022 May;23(5):791-801. doi

Insert

loxP-shuffledORF-loxP-Katushka

Use

Lentiviral

Promoter

CMV

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase-cTF

Plasmid

#87743

Purpose

IPTG-inducible expression of ligase-cTF fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and cTF (chimeric transcription factor base…

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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NLS pEGFP-N3 (1299)

Plasmid

#62043

Purpose

mammalian expression of NLS-EGFP

Depositor

Eric Schirmer

Article

Zuleger et al Genome Biol. 2013 Feb 15;14(2):R14. doi:

Insert

SV40 NLS

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pLYS1-EMRE/RNAi resistant

Plasmid

#50056

Purpose

Expression of human EMRE in mammalian cells with silent mutation making it resistant to RNAi

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

EMRE-RNAi resistant (SMDT1 Human)

Expression

Mammalian

Mutation

RNAi resistant, silent mutant

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs_NMNAT3-FLAG

Plasmid

#133261

Purpose

Express FLAG-tagged NMNAT3 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

NMNAT3 (NMNAT3 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-H2B-Dendra

Plasmid

#140559

Purpose

Red to green photoconvertible chromosome marker

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Insert

H2B

Tags

Dendra

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pTracer_Cr-TRP1

Plasmid

#64881

Purpose

Expresses CrTRP1 in mammalian cells

Depositor

Sebastián Brauchi

Article

Arias-Darraz et al Plant Cell. 2015 Jan;27(1):177-88. doi:

Insert

Transient receptor potential 1

Tags

eGFP

Promoter

CMV

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only
pFastBac1-OSF-TRIM5alpha(human)

Plasmid

#79034

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(human) (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available Since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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pHLP701

Plasmid

#203743

Purpose

C-terminal tagging of target proteins with 3xV5-IAA17 degron

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

IAA17 auxin binding domain

Use

Pcr amplification template for gene tagging

Tags

3xV5

Promoter

none

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-FNTA-FLAG

Plasmid

#86858

Purpose

To express FNTA with a FLAG tag at C terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

FNTA (FNTA Human)

Tags

FLAG

Expression

Mammalian

Mutation

The sequence of the insert is isoform 2, which di…

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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Human RNF149-3X MYC

Plasmid

#225333

Purpose

Lentiviral expression of human RNF149 with 3x myc tag in mammalian cells

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF149 (RNF149 Human)

Use

Lentiviral

Tags

3xMyc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-CMP-PE-V1

Plasmid

#202769

Purpose

Expresses CMP-PE-V1 in mammalian cells for prime editing

Depositor

Kyoungmi Kim

Article

Park et al Genome Biol. 2021 Jun 3;22(1):170. doi:

Insert

CMP-PE-V1

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-PCBP1

Plasmid

#16177

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Poly(rC) binding protein 1 (PCBP1 Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pENTR223-BORF1

Plasmid

#195804

Purpose

Gateway Entry Clone of Epstein-Barr virus BORF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BORF1

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-ABE-CT

Plasmid

#112876

Purpose

AAV inverted terminal repeat based vector plasmid encoding the C-terminal half of nCas9

Depositor

Jin-Soo Kim

Article

Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

Insert

ABE-CT

Use

AAV

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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