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pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C

Plasmid

#146071

Purpose

Insect Expression of DmTral-LSmR21EE23KR42EF44A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSmR21EE23KR42EF44A (tral Fly)

Expression

Insect

Mutation

R21E, E23K, R42E, F44A. Additionally, one silent …

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-R42EF44A_C

Plasmid

#146061

Purpose

Insect Expression of DmTral-R42EF44A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-R42EF44A (tral Fly)

Expression

Insect

Mutation

R42E, F44A. Additionally, two silent mutations A1…

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

Plasmid

#146049

Purpose

Insect Expression of DmTral-LSmR21EE23KS13AI15A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSmR21EE23KS13AI15A (tral Fly)

Expression

Insect

Mutation

S13A, I15A, R21E, E23K, one silent mutation A198C…

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C

Plasmid

#146039

Purpose

Insect Expression of DmTral-FDF-ADA

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmTral-FDF-ADA (tral Fly)

Expression

Insect

Mutation

FDF to ADA (407-409) mutation. Additionally, thre…

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti CGIP + Z-AAT target

Plasmid

#86007

Purpose

lenti reporter plasmid with Z-AAT-specific gRNA target-sequence, to be used with tGFP #26864

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Inserts

CAG promoter
Z-AAT target

Use

Lentiviral

Mutation

V30M

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti CGIP + TTR target

Plasmid

#86009

Purpose

lenti reporter plasmid with TTR_V30M-specific gRNA target-sequence, to be used with tGFP #26864

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Inserts

CAG promoter
TTR target

Use

Lentiviral

Mutation

V30M

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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GIV-FLAG L249P (LP)

Plasmid

#86097

Purpose

Expression GIV in mammalian cell

Depositor

Pradipta Ghosh

Article

Aznar et al Elife. 2016 Nov 4;5. pii: e20795. doi: 1

Insert

GIV/Girdin (CCDC88A Human)

Tags

3xFlag

Expression

Mammalian

Mutation

L249P

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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NKP9 pmRFP-N2 (719)

Plasmid

#61999

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

C17orf62 (CYBC1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
NKP40 RFP (794)

Plasmid

#62006

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

C17orf32 (C17orf32 Human)

Tags

mRFP

Expression

Mammalian

Promoter

pgk

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
STT3A pmRFP (663)

Plasmid

#61997

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

STT3A (STT3A Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
Pvalb-2A-Cre targeting vector

Plasmid

#61570

Purpose

Target the Cre recombinase gene to the stop codon of the mouse Pvalb gene

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Pvalb exon 4 - 2A - Cre

Use

Cre/Lox and Mouse Targeting

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2

Plasmid

#223149

Purpose

Expression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSSRB274_pAC8-eGFP-hsCRBN

Plasmid

#218791

Purpose

Insect cell expression vector for FLAG-TEV-eGFP-Prescission-hsCRBN

Depositor

Eric Fischer

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

Protein cereblon (CRBN Human)

Tags

FLAG-TEV-eGFP-Prescission

Expression

Insect

Mutation

eGFP fused to N-terminus

Promoter

polyhedrin

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSSRB069_pAC8-hsDDB1dB

Plasmid

#218790

Purpose

Insect cell expression vector for 6xHis-TEV-hsDDB1∆B

Depositor

Eric Fischer

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

DNA damage-binding protein 1 (DDB1 Human)

Tags

6xHis-TEV

Expression

Insect

Mutation

Deleted amino acids 396-705 and replaced with GNG…

Promoter

polyhedrin

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSDC803_IKZF1_mutant

Plasmid

#224113

Purpose

Insect cell expression vector for IKZF1 mutant for CRBN cryoEM pre-blotting

Depositor

Eric Fischer

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

IKZF1 (IKZF1 Human)

Tags

Flag-TEV

Expression

Insect

Mutation

Residues 140-196, Q146A and G151N

Promoter

polyhedrin

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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10xHis-3C-BRD4(42-459) BD1-BD2

Plasmid

#233886

Purpose

pRSF-DUET1 for protein overexpression of human BRD4

Depositor

Alessio Ciulli

Article

Hsia et al Nature. 2024 Mar;627(8002):204-211. doi:

Insert

BRD4 (BRD4 Human)

Tags

10x his 3C

Expression

Bacterial

Promoter

T7

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

Plasmid

#65458

Purpose

Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

tTA2

Use

AAV

Promoter

CAG

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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BCL6-GFP in Artichoke

Plasmid

#169931

Purpose

Overexpression of Full Length of BCL6-GFP fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

Insert

BCL6 (BCL6 Human)

Use

Lentiviral

Tags

GFP

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-DreO-bGHpA

Plasmid

#50363

Purpose

Can be used to generate AAV virus that will express DreO recombinase in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Has Service

AAV Retrograde and AAV5

Insert

DreO recombinase

Use

AAV

Tags

none

Mutation

Sequence optimized for expression in mammalian ce…

Promoter

hSyn1

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2

Plasmid

#223159

Purpose

Expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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