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pFA6a-Pho8∆60-HphMX6

Plasmid

#240973

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HphMX6 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-C.a.URA3

Plasmid

#240970

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; C.a.URA3 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8d∆60-TRP1

Plasmid

#240968

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; TRP1 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-K.I.LEU2

Plasmid

#240969

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; K.l.LEU2 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-NatMX6

Plasmid

#240972

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; NatMX6 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pYO286

Plasmid

#235730

Purpose

Protein expression of yeast VPS15 (1-981)-ZZ in yeast

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

VPS15

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

aa 1-981

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-BleMX6

Plasmid

#240974

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; BleMX6 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

BleMX6

Expression

Bacterial

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-His3MX6

Plasmid

#240971

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HIS3 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCI_Fsx1-V5_GFPnes

Plasmid

#240503

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Moi et al Nat Commun. 2022 Jul 6;13(1):3880. doi:

Insert

Fsx1

Tags

GFPnes and V5

Expression

Mammalian

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-DIO-Flpo

Plasmid

#237222

Purpose

For Cre-dependent expression of Flp recombinase

Depositor

Thomas Hnasko

Article

Oriol et al bioRxiv [Preprint]. 2025 Jan 17:2024.06.

Insert

Flpo recombinase

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hSyn

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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YIplac204-TDH3p-CDC19-CYC1t

Plasmid

#216122

Purpose

Yeast integrative vector for constitutive expression of CDC19 at the TRP1 locus

Depositor

Yvonne Nygård

Article

Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

Insert

Pyruvate kinase 1

Expression

Yeast

Promoter

TDH3 promoter

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAM72

Plasmid

#227622

Purpose

pETDuet-1 Rpn8(1-179)-precission-Strep, H6-precission-Rpn11(2-239, G77P)

Depositor

Andreas Martin

Article

Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

Inserts

Rpn11 (RPN11 Budding Yeast)
rpn8 (RPN8 Budding Yeast)

Tags

His-Prescission

Expression

Bacterial

Mutation

1-179 and aa 2-239, G77P

Promoter

T7

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pYAMTr2GCsgSeLEU2

Plasmid

#224870

Purpose

Disruption of S. eubayanus type LEU2 genes

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTr2GC having the guide sequence SeLEU2 (DI49_0736 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pYAMTrGCsgScLEU2

Plasmid

#224869

Purpose

Disruption of S. cerevisiae type LEU2 genes

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTrGCas having the guide sequence ScLEU2 (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pBWB507

Plasmid

#209325

Purpose

p15A-tetR-tet-ME-RBS-mRFP-ME-U1-Barcode-U2-dbI terminator-oriT-Cm. Plasmid backbone for fragment expression.

Depositor

Adam Arkin

Article

Biggs et al Mol Syst Biol. 2024 Oct 7. doi: 10.1038/

Insert

mRFP

Expression

Bacterial

Promoter

tet

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pYAMTr2GCsgScLEU2

Plasmid

#224868

Purpose

Disruption of S. cerevisiae type LEU2 gene

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTr2GC having the guide sequence ScLEU2 (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-APP-CTF

Plasmid

#181732

Purpose

Galactose inducible expression of APP-CTF

Depositor

Alejandro Chavez

Insert

APP-CTF (APP Human)

Expression

Yeast

Mutation

C-terminal fragment (aa 671-770)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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BcC _pET29b

Plasmid

#215403

Purpose

Expression construct for BcC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

BcC

Expression

Bacterial

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pITESceIcmd

Plasmid

#218288

Purpose

Introduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1

Depositor

Claudia Vickers

Article

McDonnell et al bioRxiv 2024.03.26.586389

Insert

HO(-253, -1)-tSynth3

Expression

Yeast

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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OoH_pET29b

Plasmid

#215424

Purpose

Expression construct for OoH gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

OoH

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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