We narrowed to 32,141 results for: Eng

1. pJT9R

   Plasmid

   #98305

   Purpose

   Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   TRPL3AcNES1>TRPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAV8-cPE-C

   Plasmid

   #183539

   Purpose

   Deliver a split compact Prime Editor in vivo

   Depositor

   Wen Xue

   Article

   Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

   Insert

   C-terminal fragment of SpCas9 nickase (H840A), Reverse Transcriptase
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U1A

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA

   Plasmid

   #74513

   Purpose

   AAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide.

   Depositor

   Guoping Feng

   Article

   Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

   Insert

   EGFP-p2A-EGFP-f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV8-cPE-N

   Plasmid

   #183538

   Purpose

   Deliver a split compact Prime Editor in vivo

   Depositor

   Wen Xue

   Article

   Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

   Insert

   N-terminal fragment of SpCas9 nickase (H840A)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. dpYPQ230-D156R-ABE8

   Plasmid

   #195569

   Purpose

   dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GSAGSAAGSGEF)x3

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

   Insert

   ecTadA8e-(GSAGSAAGSGEF)x3-dLbCas12a-D156R
   

   Use

   CRISPR; Gateway compatible ectada8e-(gsagsaagsgef…

   Tags

   5' and 3' NLS

   Expression

   Plant

   Mutation

   Promoter

   ZmUBI1

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pdCASclos

   Plasmid

   #73640

   Purpose

   Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824

   Depositor

   Sheng Yang

   Article

   Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

   Inserts

   dCas9
   sgRNA to spo0A
   

   Use

   E.coli-clostridium shuttle vector

   Tags

   Expression

   Mutation

   D10A, H840A

   Promoter

   Pj23119 and ptb

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. dpYPQ230-D156R-CBE2

   Plasmid

   #195368

   Purpose

   dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

   Insert

   hA3A/Y130F-XTEN-dLbCas12a-D156R-UGI
   

   Use

   CRISPR; Gateway compatible ha3a/y130f-xten-dlbcas…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   ZmUBI1

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-LAP2-mCherry

   Plasmid

   #159524

   Purpose

   Expresses mCherry from LAP2 promoter

   Depositor

   Esteban Engel

   Article

   Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LAP2

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHDR-CDKN2A-Ex2-CMV-EGFP

   Plasmid

   #110734

   Purpose

   Recombination template for replacing CDKN2A Exon2 with CMV-EGFP

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   EGFP
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pILGFP92

    Plasmid

    #83549

    Purpose

    Used to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TDH3 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAsCpf1(TYCV)(BB) (pY211)

    Plasmid

    #89352

    Purpose

    Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K607R

    Promoter

    CBh

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pILGFPE9

    Plasmid

    #83545

    Purpose

    Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TEF1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EF.deltaBHES1.Ubc.GFP

    Plasmid

    #24982

    Depositor

    Linzhao Cheng

    Article

    Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

    Insert

    HES1 (HES1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    DNA-binding domain disrupted by E43A, K44A, R47A …

    Promoter

    Available since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pJH4542

    Plasmid

    #200779

    Purpose

    Ptwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptune

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    GCaMP6
    

    Use

    Tags

    mNeptune

    Expression

    Worm

    Mutation

    Promoter

    Ptwk-40

    Available since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. dpYPQ230-D156R-ABE4

    Plasmid

    #195370

    Purpose

    dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

    Insert

    ecTadA8e-(GGGGS)x6-dLbCas12a-D156R
    

    Use

    CRISPR; Gateway compatible ectada8e-(ggggs)x6-dlb…

    Tags

    5' and 3' NLS

    Expression

    Plant

    Mutation

    Promoter

    ZmUBI1

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CCR5_gRNA_pX459

    Plasmid

    #192268

    Purpose

    gRNA expression for CRISPR Genome Editing of CCR5

    Depositor

    Shantanu Chowdhury

    Article

    Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

    Insert

    CCR5 guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2-sod1

    Plasmid

    #107594

    Purpose

    Contains zebrafish sod1 gene in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    sod1 (sod1 Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL3-B-pGAS5

    Plasmid

    #46371

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

    Insert

    GAS5 (GAS5 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    promoter

    Promoter

    Available since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MMLV-RT for Split-PE (pEH45)

    Plasmid

    #190105

    Purpose

    Expresses MMLV-RT (full-length, from PE2) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    MMLV-RT
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    RT pentamutant from PE2 (D200N, T306K, W313F, T33…

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pILGFP95

    Plasmid

    #83550

    Purpose

    Used to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    PDA1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PDA1

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only