We narrowed to 166,096 results for: addgene

1. AAV-CAG-flex-lck-smV5

   Plasmid

   #196423

   Purpose

   Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter; no cell-type specificity

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Insert

   Lck-smV5
   

   Use

   AAV and Cre/Lox

   Tags

   Lck

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Marathon-RT tetramutant for Split-PE (pEH101)

   Plasmid

   #190109

   Purpose

   Expresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   Marathon-RT
   

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   D14R, D74R, N116K, N197R

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. NLS-HA-tdMCP-tdTomato

   Plasmid

   #183938

   Purpose

   tandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tag

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   tdMCP-tdTomato (cp Synthetic)
   

   Tags

   NLS-HA

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. FEN1 C10.3 gRNA

   Plasmid

   #90689

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human FEN1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   FEN1 (Guide Designation C10.3)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCNL-N1_vinculin

   Plasmid

   #65691

   Purpose

   Expresses Vinculin-CNL in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   vinculin (Vcl Mouse)
   

   Use

   Luciferase

   Tags

   CNL

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pFUW-TetO-mCherry-cNLS

   Plasmid

   #138531

   Purpose

   Lentiviral inducible expression of mCherry with nuclear localization signal

   Depositor

   Christopher Grigsby, Ana Teixeira

   Article

   Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   3x SV40 cNLS

   Expression

   Mammalian

   Promoter

   TRE-CMV

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMX-Flag-WIP

   Plasmid

   #11772

   Depositor

   Jack Strominger

   Article

   Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

   Insert

   WIP (WIPF1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pAGK112

   Plasmid

   #228127

   Purpose

   Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
   

   Expression

   Mammalian

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. SMC6 G4.4 gRNA

   Plasmid

   #90899

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SMC6

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   SMC6 (Guide Designation G4.4)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. SMC6 G3.4 gRNA

    Plasmid

    #90898

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SMC6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SMC6 (Guide Designation G3.4)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. HLCS_pCSdest

    Plasmid

    #53803

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    HLCS (HLCS Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pFH6

    Plasmid

    #86555

    Purpose

    Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency

    Depositor

    Andreas Weber

    Article

    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

    Insert

    U6-26p::sgRNA scaffold
    

    Use

    CRISPR; Subcloning

    Promoter

    Arabidopsis U6-26 promoter

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pStr-KDEL_APV-SBP-DsRed2-ER-5

    Plasmid

    #170675

    Purpose

    Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Inserts

    APV-SBP-DsRed2-ER-5
    streptavidin-KDEL
    

    Expression

    Mammalian

    Promoter

    IRES and T7

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. FEN1 C9.3 gRNA

    Plasmid

    #90688

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FEN1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    FEN1 (Guide Designation C9.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGImC_Empty

    Plasmid

    #92015

    Purpose

    Empty backbone, includes IRES-mCherry

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Type

    Empty backbone

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. PCP4_pCSdest

    Plasmid

    #53896

    Purpose

    Expression of PCP4

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    PCP4 (PCP4 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pBABE puro mouse IRS-4

    Plasmid

    #11363

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-4 (Irs4 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

18. BRCA1 A9.2 gRNA

    Plasmid

    #90548

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRCA1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BRCA1 (Guide Designation A9.2)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLIK 3xFLAG-TWIST2 neo

    Plasmid

    #197936

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-tagged TWIST2

    Depositor

    Kevin Janes

    Article

    Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

    Insert

    TWIST2 (TWIST2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. BPK1169: CAG-DmrC-NLS-3xFLAG-P65

    Plasmid

    #104564

    Purpose

    Mammalian expression vector for DmrC fused to p65 activation domain

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    DmrC-p65
    

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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