We narrowed to 26,561 results for: NOL

1. pSTAP-seq_human_spike-in

   Plasmid

   #125152

   Purpose

   Empty vector to clone and measure the expression of promoter candidates (spike-in) by determining the abundance of transcripts originating from the candidate in human cells

   Depositor

   Alexander Stark

   Article

   Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

   Type

   Empty backbone

   Use

   Stap-seq screening vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330A_FokI-1x2

   Plasmid

   #63602

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTW194b63

   Plasmid

   #136930

   Purpose

   Directed evolution of SpCas10

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   npuC-gIII(C)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJ211_WT_hi

   Plasmid

   #128850

   Purpose

   High copy number plasmid for expression of E. coli flagellin from its natural promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G03 RAD54B

   Plasmid

   #67124

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   RAD54B (RAD54B Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pMM0553

   Plasmid

   #128989

   Purpose

   LEU2 integration vector of pZF(3BS)-mRUBY2

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   Leu2 5' homology-pZiF promoter-mRuby2-tADH1-Con1-LEU2-Leu2 3' homology
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLH-sgRNA1-boxB-MS2

   Plasmid

   #75396

   Purpose

   sgRNA1-boxB-MS2

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-boxB-MS2
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB4848

   Plasmid

   #106138

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCR1054

   Plasmid

   #71822

   Purpose

   Express Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLS

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. psAAVS1-T2

    Plasmid

    #60543

    Purpose

    GFP reporter for CRISPR/CAS9 and gRNA_AAVS1-T2 activities

    Depositor

    William Simonds

    Article

    Zhang et al J Biotechnol. 2014 Sep 2. pii: S0168-165

    Insert

    ProtoSpacer AAVS1-T2 (AAVS1 Human)
    

    Use

    CRISPR; Kan

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR11 mSTIM1 myc

    Plasmid

    #17733

    Depositor

    Anjana Rao

    Article

    Oh-Hora et al Nat Immunol. 2008 Apr . 9(4):432-43.

    Insert

    STIM1 (Stim1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

12. GWF196

    Plasmid

    #133613

    Purpose

    NS3aH1-VPR

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    NS3aH1-VPR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC-DEST-R1R2

    Plasmid

    #141056

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBT271.359

    Plasmid

    #127845

    Purpose

    clone A3A CBEs by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    A3A
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMAD-Gate2

    Plasmid

    #25941

    Depositor

    Kamil Onder

    Article

    Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

    Insert

    
    

    Use

    Tags

    VP16 Fusion Protein

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBT138b-pro1

    Plasmid

    #138513

    Purpose

    P3h

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET23a-LIR-1 D1-D2

    Plasmid

    #12103

    Depositor

    Pamela Bjorkman

    Article

    Willcox et al Nat Immunol. 2003 Sep . 4(9):913-9.

    Insert

    Leukocyte immunoglobulin-like receptor 1 (LILRB1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Amino acids 1−198*

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pKSB-611U6-sgRNA3 AGAG AACA

    Plasmid

    #173673

    Purpose

    To clone and express a gRNA in Anopheles

    Depositor

    Eric Marois

    Article

    Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONRPEX18Gm-SceI-pheS

    Plasmid

    #111609

    Purpose

    Gateway compatible allelic exchange vector for Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Type

    Empty backbone

    Use

    Allelic exchange in burkholderia sp.

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCBEclos-cbei2385-g2-opt

    Plasmid

    #118215

    Purpose

    CRISPR-mediated base editing in Clostridium

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol Bioeng. 2019 Feb 10. doi: 10.

    Insert

    Apobec1, Casp nickase, UG1-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptm

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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