We narrowed to 67,707 results for: nin

1. pPbuCas13b

   Plasmid

   #184565

   Purpose

   Inducible expression of PbuCas13b nuclease in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Cas13b nuclease from Prevotella buccae
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQA-LP574

   Plasmid

   #222953

   Purpose

   Expresses R120A LoaP

   Depositor

   Adrian Ferré-D'Amaré

   Article

   Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

   Insert

   antiterminator protein LoaP
   

   Use

   Tags

   10xHIS-SUMO

   Expression

   Bacterial

   Mutation

   mutated Arginine 120 to Alanine

   Promoter

   T7

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS2712-CMV-eNme2-C-ABE8e-i1-L10

   Plasmid

   #228733

   Purpose

   Expresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid eNme-C nCas9 (D16A)-i1-L10
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSiMPlc_N + pSiMPlc_C

   Plasmid

   #134312

   Purpose

   Maintenance of 2 plasmids with a single antibiotic in E. coli

   Depositor

   Barbara Di Ventura

   Article

   Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

   Insert

   cmR_gp41-1 N-intein + cmR_gp41-1 C-intein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. CROP_C9_Puro_DNMT3B

   Plasmid

   #183284

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets DNMT3B gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   DNMT3B
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEMT-PT2A-iRFP670-Tdtomato-GFP

   Plasmid

   #111817

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   iRFP670
   Tdtomato
   GFP
   

   Use

   Cloning intermediate

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. SLC30A10-GFP

   Plasmid

   #104381

   Purpose

   Expresses SLC30A10-GFP wild-type in mammalian cells

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Selyunin et al J Cell Biol. 2017 Oct 2;216(10):3249-326

   Insert

   SLC30A10 (SLC30A10 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOD_C3006

   Plasmid

   #91096

   Purpose

   Module C, Promoter: FMV 34S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   GFP (+ BaeI site for GT donor cloning)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   FMV 34S

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIIS18-BsaI

   Plasmid

   #128649

   Purpose

   Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMOD_C3012

    Plasmid

    #91099

    Purpose

    Module C, Promoter: PvUbi1, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    GFP (+ BaeI site for GT donor cloning)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    PvUbi1

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pnxABE-flgn

    Plasmid

    #187932

    Purpose

    A-to-G base editor

    Depositor

    Yi Xiao

    Article

    et al ACS Synth Biol. 2022 Apr 15;11(4):1650-1

    Insert

    ecTadA(8e)-nxCas9 (D10A)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SpRY

    Plasmid

    #198544

    Purpose

    Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpRY
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-nlsGFP-P2A

    Plasmid

    #80808

    Purpose

    nuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2A

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    minimal cytomegalovirus immediate early enhancer/promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNL4-3 envFS eGFP NoStarts

    Plasmid

    #101342

    Purpose

    All ATGs from the start of gag to NC mutated to ATC except the first which was mutated to ACG

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    No methonines in Gag

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC-CR7T-gRNA2.1-nlsBFP

    Plasmid

    #170515

    Purpose

    gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CR7T

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Accessory plasmid 2

    Plasmid

    #128571

    Purpose

    for cloning identified individual SPECS

    Depositor

    Timothy Lu

    Article

    Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagRFP

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. CROP_C9_Puro_DNMT3A

    Plasmid

    #183283

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets DNMT3A gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    DNMT3A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKB01_mOrange2

    Plasmid

    #69102

    Purpose

    Expresses mOrange2 (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

    Depositor

    Jan-Willem Veening

    Article

    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

    Insert

    mOrange2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. CROP_C9_Puro_PARP1

    Plasmid

    #183312

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets PARP1 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    PARP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lentiviral-FKBP-cpGFP-Nb39

    Plasmid

    #191498

    Purpose

    Rapamycin-dependent opioid sensor for opioid receptors (component 2)

    Depositor

    Wenjing Wang

    Article

    Kroning et al ACS Chem Biol. 2022 Aug 19;17(8):2212-22

    Insert

    FKBP-cpGFP-Nb39
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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