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Plasmid#118026PurposeAAV vector for sparse and bright labeling in a cell-type specific mannerDepositorInsertTRE-fDIO-GFP-IRES-tTA
UseAAVTagsExpressionMutationSee depositor comments belowPromoterTREAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAPZa-STE2-1D4
Plasmid#133422PurposeMutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.DepositorInsertSTE2 (STE2 Budding Yeast)
UseTagsExpressionYeastMutationC59S, C252APromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_human_ADAR1_ccB
Plasmid#158116Purposedeletion hADAR1DepositorInsertAdar1 (ADAR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL-MS2-12X
Plasmid#27119DepositorInsertMS2 binding sites (12)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFUGW SpCas9
Plasmid#131506Purposelentiviral expression of SpCas9 under hUBC promotorDepositorInsertSpCas9
UseLentiviralTagsExpressionMammalianMutationPromoterhUBCAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-tdTomato (AAV8)
Viral Prep#28306-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (Cre-dependent)Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_12GLI-FLuc_R4-R3
Plasmid#113712PurposeSingle pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.DepositorInsert12GLI-FLuc
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-C4-Nrf2
Plasmid#21549DepositorInsertnuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)
UseTagsEGFP and MycExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC G3BP1-GFP-GFP
Plasmid#119950PurposeLentiviral vector expressing G3BP1-GFP fusion proteinDepositorInsertG3BP1 (G3BP1 Human)
UseLentiviralTagsGFPExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-UbE2D3
Plasmid#186805PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.DepositorInsertHis-Tev-UbE2D3 (UBE2D3 Human)
UseTagsHis6-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXR004: CasRx pre-gRNA cloning backbone
Plasmid#109054PurposehU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSIRV-NF-kB-eGFP
Plasmid#118093PurposeFlourescent reporter for NF-kB activityDepositorInserteGFP
UseRetroviralTagsExpressionMutationPromoterminimal promoter with NF-kB responsive elementAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
HRE-luciferase
Plasmid#26731PurposeLuciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.DepositorInsertHypoxia Responsive Elements (3)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBa-LSS-GFP-LDLR wt
Plasmid#98184PurposeLow Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.DepositorInsertLDLR (LDLR Human)
UseTagsGFP and LDLR signal sequence, N-term of GFP so GF…ExpressionMammalianMutationnonePromoterChicken Beta ActinAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-sfGFP 150TAG
Plasmid#85493PurposepET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
UseTagsExpressionBacterialMutation150 TAG (amino acid 150 mutated to stop codon)PromoterT7Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-sumo-NSP12 (SARS-CoV-2)
Plasmid#159107PurposeThe SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).DepositorInsertNSP12
UseTagsHis-SUMO tagExpressionBacterialMutationNonePromoterT7Available sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmVen-RPA70-IRES-RPA32-IRES-RPA14
Plasmid#211431Purposemammalian expression of human RPA70 N-terminally fused to mVenus and RPA32 and RPA14DepositorUseTagsmVenus (on RPA70)ExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Cyclin A2
Plasmid#215048PurposeExpresses EGFP-Cyclin A2 in mammalian cells from a retroviral vector.DepositorInsertCCNA2 (CCNA2 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterLTRAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP13
Plasmid#22568DepositorInsertUSP13 (USP13 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceDec. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-RhoA-Q63L
Plasmid#12968DepositorInsertRhoA constitutively active (RHOA Human)
UseTagsEGFPExpressionMammalianMutationQ63L Constitutively activePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only