We narrowed to 13,665 results for: crispr cas9

1. pENTR-R4R3-2xmEGFP-C

   Plasmid

   #113750

   Purpose

   Gateway multi-site entry clone for second position 2x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   mEGFP-mEGFP
   

   Use

   CRISPR; Gateway entry vector

   Tags

   2xmEGFP

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-R4R3-2xmRuby-N

   Plasmid

   #113753

   Purpose

   Gateway multi-site entry clone for second position 2x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   mRuby2-mRuby2
   

   Use

   CRISPR; Gateway entry vector

   Tags

   2xmRuby2

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-R4R3-2xmRuby-C

   Plasmid

   #113756

   Purpose

   Gateway multi-site entry clone for second position 2x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   mRuby2-mRuby2
   

   Use

   CRISPR; Gateway entry vector

   Tags

   2xmRuby2

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUDP082

   Plasmid

   #103875

   Purpose

   Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUDP025

   Plasmid

   #103874

   Purpose

   Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactis

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUDP013

   Plasmid

   #103873

   Purpose

   Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PE2-P2A-PuroR

   Plasmid

   #196962

   Purpose

   Expresses PE2 with puromycin resistance

   Depositor

   Leopold Parts

   Insert

   PE2-P2A-Puro
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSD_FRB-TALETS3-3xFLAG-2xNLS

   Plasmid

   #107306

   Purpose

   Expresses TALE-VEGFA-TS3 fused to FRB in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   TALE_VEGFA-TS3
   

   Use

   CRISPR

   Tags

   3x Flag, 2xNLS and FRB

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBM019

   Plasmid

   #128179

   Purpose

   Construct for generating stable Cas9-expressing Toxoplasma gondii

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

   Inserts

   sgRNA#1
   SpCas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3X FLAG and Nuclear Localization Signal

   Promoter

   TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3-Basic-8x-gRNA-eGFP

    Plasmid

    #60718

    Purpose

    eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJH375

    Plasmid

    #86841

    Purpose

    mamalian expression of anti-CRISPR protein AcrIIA3

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRAU168

    Plasmid

    #86834

    Purpose

    bacterial expression of anti-CRISPR protein AcrIIA1

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA1
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. BCJJ125

    Plasmid

    #117515

    Purpose

    Cas9_3 (with intron) Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:AATG:Cas9_3(intron):GCTT:BsaI
    

    Use

    CRISPR

    Tags

    2xFLAG and NLS

    Expression

    Plant

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSW21

    Plasmid

    #86836

    Purpose

    bacterial expression of anti-CRISPR protein AcrIIA4

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA4
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRAU171

    Plasmid

    #86835

    Purpose

    bacterial expression of anti-CRISPR protein AcrIIA2

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA2
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRA03

    Plasmid

    #103141

    Purpose

    Triple gRNA expression separated with 28nt Csy4 motif

    Depositor

    Jens Nielsen

    Article

    Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

    Insert

    gRNAs
    

    Expression

    Yeast

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. BCJJ180

    Plasmid

    #117516

    Purpose

    Cas9_4 Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:AATG:Cas9_4:GCTT:BsaI
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Plant

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICSL90016

    Plasmid

    #117514

    Purpose

    Cas9_2 Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:AATG:Cas9_2:GCTT:BsaI
    

    Use

    CRISPR

    Tags

    NLS_SV40

    Expression

    Plant

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. U6>XX

    Plasmid

    #87542

    Purpose

    Component 1 of 2 for U6>sgRNA cassette OSO-PCR

    Depositor

    Lionel Christiaen

    Article

    Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

    Type

    Empty backbone

    Available Since

    Jan. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMLS719

    Plasmid

    #188894

    Purpose

    miniMos Arm sgRNA expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    PU6(K09B11.12)::miniMos Arm sgRNA
    

    Expression

    Worm

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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