We narrowed to 79,907 results for: ANT

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pYPQ-dSpRY-Act3.0

Plasmid

#158416

Purpose

CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pUTKan-3xHA-GFP-TOM5

Plasmid

#130674

Purpose

Ubiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial membrane of planta

Depositor

Andreas Weber

Article

Kuhnert et al Plant Physiol. 2020 Feb;182(2):692-706.

Insert

Translocase of outer mitochondrial membrane 5 (TOM5 Mustard Weed)

Tags

3xHA and sGFP

Expression

Plant

Promoter

Ubiquitin10

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_C2515

Plasmid

#91083

Purpose

Module C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

AtU6

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET30a-SARS-CoV2-NP

Plasmid

#165090

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)

Expression

Bacterial

Available Since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAT-WT-HA-GFP11

Plasmid

#177719

Purpose

ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Tags

GFP11 and HA

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-dzCas9-NG-Act3.0

Plasmid

#158415

Purpose

CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A

Plasmid

#110555

Purpose

pFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.

Depositor

Martin Pule

Article

Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

Insert

Anti-RD114 heavy chain

Tags

mIgG2A

Expression

Bacterial and Mammalian

Promoter

EF-1 alpha core promoter

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET30a-Spike-E

Plasmid

#165096

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)

Expression

Bacterial

Available Since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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6691 Bicistronic_ires_puro

Plasmid

#64335

Purpose

This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Type

Empty backbone

Use

Retroviral

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSB161 - pL2_pSB90_2x35S::GUS::tMAS

Plasmid

#123197

Purpose

binary plant vector for transient expression of GUS (with introns)

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::GUS::tMAS

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_B2203

Plasmid

#91066

Purpose

Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE C

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers

Use

CRISPR

Promoter

CmYLCV

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_B2112

Plasmid

#91064

Purpose

Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers

Use

CRISPR

Promoter

PvUbi1

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)

Plasmid

#160611

Purpose

Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

P35s:LbCas12a:T35s

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD_B2101

Plasmid

#91060

Purpose

Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers

Use

CRISPR

Promoter

35S

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ-SpRY-Act3.0

Plasmid

#178957

Purpose

It consists of a catalytically active SpRY nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Insert

SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_B2103b

Plasmid

#91062

Purpose

Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers

Use

CRISPR

Promoter

CmYLCV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_C2518

Plasmid

#91087

Purpose

Module C, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

TaU6

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB120

Plasmid

#68574

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD_B2301

Plasmid

#91067

Purpose

Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers

Use

CRISPR

Promoter

35S

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pSB97 - pL2_pVS1_2x35S::fLUC-I::tNOS

Plasmid

#123189

Purpose

binary plant vector for transient fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::fLUC-I::tNOS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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