We narrowed to 11,028 results for: HA

1. pRV∆L-5Flpo

   Plasmid

   #182965

   Purpose

   The 3rd generation of rabies vector expressing Flpo recombinase

   Depositor

   Ian Wickersham

   Article

   Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   Flpo recombinase
   

   Use

   Rabies

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-6P-HMP1_FABD

   Plasmid

   #186436

   Purpose

   Expresses the HMP1 F-actin Binding domain (677-927) with an N-terminal GST tag and a PRescission Protease cleavage site

   Depositor

   Tina Izard

   Article

   Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

   Insert

   HMP1 (F-actin Binding Domain) (hmp-1 Nematode)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pST39-Mega'

   Plasmid

   #216877

   Purpose

   Derived from the plasmid for Mega GVs, pST39-pNL29 (Addgene #91696), but the overlap between GvpS and GvpK has been resolved.

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Insert

   Derived from Mega, and GvpS and GvpK overlap has been resolved
   

   Expression

   Bacterial

   Available Since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. RT-4

   Plasmid

   #220443

   Purpose

   Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.

   Depositor

   Marvin Wickens

   Article

   Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

   Insert

   PUF3 (PUF3 Budding Yeast)
   

   Tags

   3xHA

   Expression

   Yeast

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMS110

   Plasmid

   #215674

   Purpose

   Split hygromycinR landing pad insertion plasmid for ChrIII

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   5'HA + synthetic guide site3 + 3'ΔHygR:unc-54 3' UTR + lox2272 + SEC + lox2272 + 3'HA
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Mutation

   3' ∆HYGR is promoterless and encodes aa60-341

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4/TO-Cdc45HA

   Plasmid

   #211427

   Purpose

   tet-inducible mammalian expression of human Cdc45 C-terminally fused to HA-Tag

   Depositor

   Helmut Pospiech

   Article

   Kohler et al Cell Cycle. 2016;15(7):974-85. doi: 10.1

   Insert

   Cdc45 (CDC45 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-WDR5-P2A-SH2-P2A-Ras-CAAX

   Plasmid

   #209712

   Purpose

   Mammailan expression of WDR5, SH2, and HRas separated by P2A.

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   WDR5-P2A-SH2-P2A-Ras-CAAX
   

   Expression

   Mammalian

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-NLS-MBP-rATOM(WDR5)

   Plasmid

   #209710

   Purpose

   Mammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signal

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   NLS-MBP-rATOM(WDR5)
   

   Tags

   NLS-MBP

   Expression

   Mammalian

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRV∆L-5tTA

   Plasmid

   #182966

   Purpose

   The 3rd generation of rabies vector expressing a tetracycline-controlled transactivator (tTA)

   Depositor

   Ian Wickersham

   Article

   Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   tTA
   

   Use

   Rabies

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6xHis(TEV)-Drp1(K569A,K571A)-StrepII

    Plasmid

    #174426

    Purpose

    Expresses human Drp1 Isoform 3 with K569A, K571A mutations in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    K569A, K571A

    Promoter

    T7

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2+-wbp2nlY282F

    Plasmid

    #172468

    Purpose

    expresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domain

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlY282F
    

    Use

    Xenopus expression

    Tags

    none

    Mutation

    changed Tyrosine at aa282 to Phenylalanine

    Promoter

    SP6

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6xHis(TEV)-Drp1(K557A,K560A)-StrepII

    Plasmid

    #174425

    Purpose

    Expresses human Drp1 Isoform 3 with K557A, K560A mutations in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    K557A, K560A

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+-wbp2nlF127P

    Plasmid

    #174507

    Purpose

    expresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domain

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlF127P
    

    Use

    Xenopus expression

    Tags

    none

    Mutation

    changed Phenylalanine at aa127 to Proline

    Promoter

    SP6

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+-wbp2nlY55F

    Plasmid

    #172466

    Purpose

    expresses protein with one aa substitution to affect potential phosphorylation in ITSM domain

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlY55F
    

    Use

    Xenopus expression

    Tags

    none

    Mutation

    changed Tyrosine at aa55 to Phenylalanine

    Promoter

    SP6

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2+-wbp2nl

    Plasmid

    #172463

    Purpose

    expresses wild-type protein when injected into embryos

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nl (wbp2nl.L Frog)
    

    Use

    Xenopus expression

    Tags

    none

    Mutation

    none

    Promoter

    SP6

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBlastR-5xQUAS-EBFP2-GMRWhite

    Plasmid

    #165921

    Purpose

    Blasticidin resistant 5xQUAS EBFP2 GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable QUAS Response Vector
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBlastR-5xQUAS-sfGFP-MiniWhite

    Plasmid

    #165922

    Purpose

    Blasticidin resistant 5xQUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable QUAS Response Vector
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBlastR-5xQUAS-EBFP2-MiniWhite

    Plasmid

    #165924

    Purpose

    Blasticidin resistant 5xQUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable QUAS Response Vector
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBlastR-5xUAS-EBFP2-MiniWhite

    Plasmid

    #165912

    Purpose

    Blasticidin resistant 5xUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. P[acman]-Alpha1

    Plasmid

    #165858

    Purpose

    Alpha1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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