We narrowed to 22,619 results for: his
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Plasmid#229457PurposeExpresses IraD with R67A mutation with His-tagDepositorInsertIraD (R67A)
Tags6xHisExpressionBacterialMutationR67APromoterT7Available SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF18
Plasmid#229456PurposeExpresses IraD (aa17-130) with His-tagDepositorInsertIraD (aa 17-130 only)
Tags6xHisExpressionBacterialMutationaa 17-130 onlyPromoterT7Available SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-DXO
Plasmid#177993PurposeFor purification of N-terminally His-tagged DXO from bacteriaDepositorAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-HO1(51-231)
Plasmid#203486PurposeFor recombinant protein production in Escherichia coli. His-tagged Arabidopsis thaliana HEME OXYGENASE 1 (HO1) without the plastid transit peptide under the control of the T7 promoter.DepositorInsertArabidopsis thaliana HEME OXYGENASE1 (51 aa – 231 aa)
ExpressionBacterial and PlantAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
eSrtA-MT4
Plasmid#200306PurposeBacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtADepositorAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-THTAP-SNX15, residues 263-342
Plasmid#180624PurposeBacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-GST tag. Internal ID:WISP21-130.DepositorAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 SNX15 MIT residues 263-342
Plasmid#180625PurposeBacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-SUMO tag. Internal ID:WISP21-14DepositorAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 KATNAL2 residues 1-98
Plasmid#180619PurposeBacterial expression for KATNAL2 , residues 1-98. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-28.DepositorInsertKATNAL2 (KATNAL2 Human)
TagsHIS-SUMOExpressionBacterialMutationResidues 1-98PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
ATX3 13Q- I77K Q78K W87K
Plasmid#185908PurposeExpresses ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-I77K, Q78K,W87K (N-terminal ) fused with His-Tag and TEV cleavage sequence (C-terminal).DepositorInsertataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track and point mutations I77K, Q78K and W87K (ATXN3 Synthetic, Human)
Tags6x His-Tag and TEV cleavage siteExpressionBacterialMutationPoint mutations in Atx3 gene I77K Q78K and W87KAvailable SinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCC_164 SpCas9 R1333A/R1335A
Plasmid#179528PurposeFor bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tagDepositorInsertSpCas9 R1333A/R1335A
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginines 1333 and 1335 to alanineAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCC_103 SpCas9 T1337C
Plasmid#179525PurposeFor bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tagDepositorInsertSpCas9 T1337C
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged threonine 1337 to cysteineAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
AlkB-D135G
Plasmid#160434PurposeA plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to GlyDepositorInsertE. coli AlkB
TagsHis-tagExpressionBacterialMutationChanged Aspartic acid 135 to Glycine; deleted the…PromoterT7Available SinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz109_CsoS2_NTD
Plasmid#140842PurposeHis-tagged CsoS2 N-terminal domain (NTD), pET-14b backboneDepositorInsertCsoS2 N-terminal domain (NTD)
TagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 262-869PromoterT7Available SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz33_CsoS2_NTD_GFP
Plasmid#140861PurposeHis-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFPDepositorInsertCsoS2 NTD fused to GFP
TagsHexahistidine tag and Superfolder GFPExpressionBacterialPromoterT7Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz110_CsoS2_CTD
Plasmid#140844PurposeHis-tagged CsoS2 C-terminal domain (CTD), pET-14b backboneDepositorInsertCsoS2 C-terminal domain (CTD)
TagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 1-594PromoterT7Available SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 δCLD
Plasmid#73876PurposeδCLD - His tagged (2α helices+μHD deleted)DepositorInsertδCLD
TagsHisExpressionYeastPromoterMet25Available SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
P415 Ret2LD
Plasmid#73875PurposeRet2LD - His tagged (2α helices+μHD deleted)DepositorInsertRet2LD
TagsHisExpressionYeastPromoterMet25Available SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)
Plasmid#153442PurposeE. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.DepositorInsertRc.XDocB
Tags3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprin…ExpressionBacterialPromoterT7 promoterAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail
Plasmid#60392PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) using a GAL promoterDepositorInsertTUB1 (TUB1 Synthetic, Budding Yeast)
ExpressionYeastMutationinternal 6xHis (see supplement figure 4), amino a…PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB
Plasmid#176587PurposeBacterial expression of His14-MBP-TEV-AmaTnpBDepositorInsertsMBP tag
TnpB
TagsHis14 tagExpressionBacterialAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only