We narrowed to 59,537 results for: cloning

1. p663-UBC-miniTurbo-V5-DYRK4_IDG-K

   Plasmid

   #158184

   Purpose

   Gateway destination clone of DYRK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-DYRK4 (DYRK4 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICSL30003

   Plasmid

   #50304

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS, no stop codon, mCherry variant of RFP (Discosoma sp.)
   

   Expression

   Plant

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBSDONR P1-P4

   Plasmid

   #71744

   Purpose

   ampicillin resistant derivative of pDONR221 P1-P4 for multisite GATEWAY cloning

   Depositor

   Roger Innes

   Article

   Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

   Type

   Empty backbone

   Use

   Gateway entry vector for use in multisite gateway…

   Available Since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xDBE_RE:NLuc

   Plasmid

   #178318

   Purpose

   FOXO NLuc luciferase reporter

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

   Insert

   FOXO NLuc Reporter
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Mammalian

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS6

   Plasmid

   #55752

   Purpose

   Produces T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   T7 RNA Polymerase
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. NM1-5S-tRNA-SgH

   Plasmid

   #128178

   Purpose

   Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

   Depositor

   Huimin Zhao

   Article

   Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

   Insert

   gRNA cloning cassette
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   5S rRNA-tRNA(Arg)

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPAGFP-C1

   Plasmid

   #11910

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

   Has Service

   Cloning Grade DNA

   Insert

   PAGFP
   

   Expression

   Mammalian

   Mutation

   These plasmids are essentially the same as the Cl…

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5E-mnx1

   Plasmid

   #82402

   Purpose

   Gateway-compatible zebrafish mnx1 promoter

   Depositor

   Judith Eisen

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   mnx1 promoter
   

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJC53.2

   Plasmid

   #26536

   Purpose

   T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes

   Depositor

   Phillip Newmark

   Article

   Collins et al PLoS Biol. 2010 . 8(10):e1000509.

   Type

   Empty backbone

   Use

   T/a cloning vector for dsrna generation and the g…

   Available Since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. mTB3-BC2 nanobody

    Plasmid

    #210208

    Purpose

    Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

    Depositor

    Brian Kay

    Article

    Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

    Insert

    BC2 Nanobody
    

    Expression

    Bacterial

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPINM

    Plasmid

    #26044

    Depositor

    Ray Owens

    Article

    Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

    Insert

    His-MBP-3C-lacZ
    

    Tags

    MAHHHHHHSSG_MBP_LEVLFQGP

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOPINE

    Plasmid

    #26043

    Depositor

    Ray Owens

    Article

    Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

    Insert

    His-lacZ-KHHHHHH
    

    Tags

    KHHHHHH and MAHHHHHHSSG

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. 4xHRE_RE:RedF

    Plasmid

    #178314

    Purpose

    HRE RedFirefly luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    HRE RedFirefly reporter
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL198

    Plasmid

    #180472

    Purpose

    For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

    Depositor

    Peggy Lemaux

    Article

    Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    ZmCas9

    Expression

    Plant

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICSL30009

    Plasmid

    #50301

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    N terminal Myc tag (4x Myc)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUPRT::DHFR-D

    Plasmid

    #58528

    Purpose

    replace TgUPRT with DHFR by homologous recombination

    Depositor

    David Sibley

    Article

    Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.

    Insert

    DHFR cassette
    

    Use

    Gateway cloning

    Mutation

    pyrimethamine resistant

    Promoter

    DHFR

    Available Since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO HIS DNAJB2a

    Plasmid

    #19550

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB2a
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICSL50010

    Plasmid

    #50310

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    C terminal Myc tag (4x Myc)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICH41421

    Plasmid

    #50339

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. MXS_CMV::rtTA3-bGHpA

    Plasmid

    #62445

    Purpose

    MXS_chaining vector with CMV::rtTA3-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the reverse tetracycline transactivator with CMV promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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