We narrowed to 150,834 results for: ins

1. pLIC-lyn11-myc-LOV2GIVe

   Plasmid

   #170623

   Purpose

   For the mammalian expression of mLOV2GIVe

   Depositor

   Mikel Garcia-Marcos

   Article

   Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

   Insert

   Lyn11-myc-LOV2GIVe
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. MSP1594

   Plasmid

   #65775

   Purpose

   Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSN007

   Plasmid

   #102440

   Purpose

   For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

   Depositor

   Alejandro Chavez, George Church

   Article

   Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

   Insert

   7SK paired gRNA insert
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. vas-Cas9.874Z

   Plasmid

   #112687

   Purpose

   Express hSpCas9 under vasa promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   hSpCas9
   

   Tags

   3x FLAG, EGFP, and NLS

   Expression

   Insect

   Mutation

   Humanized Cas9 bacterial sequence

   Promoter

   vasa

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe-PI(WT)-Src(WT)-mCherry

   Plasmid

   #87356

   Purpose

   PI(WT): Photo-inhibitable/blue light sensitive ; WT: wild type

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   Src (Src Mouse)
   

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLinker-AID-3xHA-DHFR-LoxP

   Plasmid

   #86669

   Purpose

   for generation of AID strains using CRISPR tagging technology

   Depositor

   David Sibley

   Article

   Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

   Insert

   Linker-AID-3xHA-HXGPRT 3'UTR
   

   Use

   Expression in toxoplasma gondii

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXylA-agrCA-I

   Plasmid

   #53437

   Purpose

   xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   agrCA locus
   

   Use

   Synthetic Biology

   Tags

   none

   Expression

   Bacterial

   Mutation

   "MVQTS" sequence added to the N-terminu…

   Promoter

   PxylA

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. Htb2-GFP

   Plasmid

   #117669

   Purpose

   Colorize the nuclei

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   Htb2-GFP
   

   Expression

   Yeast

   Mutation

   Contains the gene encoding the Histone Htb2 from …

   Promoter

   pGAP

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJL1 BSMT1

   Plasmid

   #106287

   Purpose

   Expresses BSMT1 in Ecoli off a T7 promoter

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   BSMT1
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLinker-AID-Ty-HXGPRT-LoxP

    Plasmid

    #86667

    Purpose

    for generation of AID strains using CRISPR tagging technology

    Depositor

    David Sibley

    Article

    Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

    Insert

    Linker-AID-Ty-HGPRT 3'UTR
    

    Use

    Expression in toxoplasma gondii

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM

    Plasmid

    #71811

    Purpose

    Generating transgenic flies for FLP-inducible DamID

    Depositor

    Bas van Steensel

    Article

    Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

    Insert

    min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-LAM
    

    Expression

    Insect

    Available Since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. MBP-His6-TEV-MMS2

    Plasmid

    #25465

    Depositor

    Cynthia Wolberger

    Article

    Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

    Insert

    MMS2 (MMS2, UBC13 Budding Yeast)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Available Since

    July 21, 2010

    Availability

    Academic Institutions and Nonprofits only

13. 1137=Hsp70Bb-Cas9[dsRed]

    Plasmid

    #149424

    Purpose

    The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Promoter

    Dmel Hsp70Bb

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_Tiam1-pMagFast2(3x)-iRFP

    Plasmid

    #67299

    Purpose

    Blue light dependent photoswitching system

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

    Insert

    Tiam1-pMagFast2(3x)-iRFP (Tiam1 Synthetic, Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open]

    Plasmid

    #71809

    Purpose

    Generating transgenic flies for FLP-inducible DamID

    Depositor

    Bas van Steensel

    Article

    Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

    Insert

    min.hsp70P-FRT-STOP#1-FRT-Dam-Myc[open]
    

    Expression

    Insect

    Available Since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAct:dCas9-VP64

    Plasmid

    #78901

    Purpose

    Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. AICSDP-144:CTCF-mEGFP

    Plasmid

    #168799

    Purpose

    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human CTCF

    Depositor

    Allen Institute for Cell Science

    Insert

    CTCF (CTCF Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    June 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pBS AluYa5rescueA70D

    Plasmid

    #78657

    Purpose

    Generates Alu inserts suitable for rescue

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

    Insert

    Ya5rescue cassette
    

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. NpT7-5-Erk2

    Plasmid

    #39230

    Depositor

    Melanie Cobb

    Article

    Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

    Insert

    Erk2 (Mapk1 Rat)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pAAV2_VP1_RaPID_r1c3

    Plasmid

    #65723

    Purpose

    Produce random mCherry insertion in AAV2 VP3 region of VP1; ITRs in VP1 only

    Depositor

    Junghae Suh

    Article

    Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

    Insert

    mCherry
    

    Use

    AAV

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only