161,398 results

1. Puro-iNgn3

   Plasmid

   #75337

   Purpose

   doxycycline inducible Ngn3 over-expression

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

   Insert

   neurogenin 3 (Neurog3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-tight

   Available since

   May 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-SLC5A2_STOP

   Plasmid

   #161407

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC5A2 (SLC5A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

   Plasmid

   #202569

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Use

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Arginine 261 to Alanine

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-BFP-U6-sgRNA

   Plasmid

   #196725

   Purpose

   Sortable sgRNA expression, Chen tracrRNA PMID: 24360272

   Depositor

   Bernhard Schmierer

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSG86-J23151-LasR

   Plasmid

   #102456

   Purpose

   Expresses LasR protein in E. coli. The expression is controlled by a constitutive promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   LasR (lasR )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23151

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJ23101

   Plasmid

   #70252

   Purpose

   Expresses YFP from J23101 promoter and CFP from standard J23101 promoter

   Depositor

   Jim Haseloff

   Article

   Grant et al Mol Syst Biol. 2016 Jan 25;12(1):849.

   Insert

   promoter J23101-RBS B0034-EYFP-B0015
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCXLE-mCherry-t2a-SOX2-p2a-KLF4

   Plasmid

   #193296

   Purpose

   episomal mCherry-hSK (expressing mCherry + human SOX2 + KLF4)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   mCherry-t2a-SOX2-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD649-HAsp-SEMA3E-COMP5AP-AviTag-9xHis

   Plasmid

   #157534

   Purpose

   Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA3E (SEMA3E Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. M4754 KanMX::HIS3 Disruptor Converter

   Plasmid

   #51684

   Purpose

   Yeast marker swap plasmid for converting KanMX3 to HIS3

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   HIS3 (HIS3 Budding Yeast)
   

   Use

   Yeast marker swap

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWS5399-CRISPRai-ZeoR

    Plasmid

    #182838

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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