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PJQ47

Plasmid

#134646

Purpose

Contains Promoter 1 with bsa1 cut sites for inserting sgRNA

Depositor

Jason Peters, Oren Rosenberg

Article

Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Anderson BBa_J23117

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pRS405-PTEF-Dcr1

Plasmid

#22314

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Oct 23;326(5952):544-550.

Insert

DCR1

Use

Integrating plasmid

Expression

Yeast

Available Since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pZa-tetO-csgA-CmR

Plasmid

#89579

Purpose

Express csgA curli protein

Depositor

Urartu Seker

Article

Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

Insert

csgA

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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GST-Ub4

Plasmid

#171421

Purpose

For the purifcation of tetra linear ubiquitin

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

Ubiquitin

Expression

Bacterial

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBBR4pemIK

Plasmid

#126928

Purpose

Broad host range vector with toxin-antitoxin stability system for maintenance in the absence of antibiotic selection

Depositor

Lindsey Burbank

Article

Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pQR

Plasmid

#48099

Purpose

Cumate-inducible expression of mCherry-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

3xFLAG and mCherry

Expression

Bacterial

Promoter

PQ5

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pBBR1-kan-Ptet-RecETh1h2h3h4_agrob6

Plasmid

#180428

Purpose

Genome engineering of Agrobacterium

Depositor

Jun Fu

Article

Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

Insert

RecETh1h2h3h4_agrob6

Expression

Bacterial

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xF-dyskerin

Plasmid

#126870

Purpose

Expresses 3xFlag dsykerin

Depositor

Kathleen Collins

Article

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

Dyskerin

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-CMV-DsRed-IRES-d4EGFP

Plasmid

#41943

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->GFPlig

Plasmid

#162223

Purpose

Lentiviral vector for constitutive expression of GFP ligand in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab13-T22N

Plasmid

#110501

Purpose

Dominant Negative

Depositor

Bernardo Mainou

Article

Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

Insert

Rab13-T22N (RAB13 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T22N

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAN1201

Plasmid

#74695

Purpose

Circuit backbone plasmid (LacZa insert)

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Type

Empty backbone

Expression

Bacterial

Available Since

June 6, 2016

Availability

Academic Institutions and Nonprofits only

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pWT019a

Plasmid

#107893

Purpose

W1.1 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1 and is part of CAMERA 1.1

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-sd2U-Cas9, PLacO-sgRNA1

Expression

Bacterial

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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Terminase_PhiV1

Plasmid

#188836

Purpose

Large terminase from phage PhiV1

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Terminase

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pgRNA_AT

Plasmid

#196243

Purpose

Backbone plasmid for cloning sgRNA

Depositor

Andrea Puhar

Article

Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3_Csx29

Plasmid

#197606

Purpose

Expresses Csx29 in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

Csx29

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Portal_PhiV1

Plasmid

#188811

Purpose

Portal protein from phage PhiV1

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Portal

Expression

Bacterial

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Terminase_ST32

Plasmid

#188850

Purpose

Large terminase from phage ST32

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Terminase

Expression

Bacterial

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT23

Plasmid

#89663

Purpose

expression of doubleTAL staple protein dT23 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT23

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pACRISPR-CM

Plasmid

#203588

Purpose

A sgRNA expression plasmid for genome editing in Burkholderia

Depositor

Leonilde Moreira

Article

Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

Type

Empty backbone

Expression

Bacterial

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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