We narrowed to 15,989 results for: LEA
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Plasmid#184976PurposeTest effect of extending a1/a2 on CAN1 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 27 v1
ExpressionYeastMutationCAN1 donor G444stop, a1/a2 length extended to 27 …PromoterGal7Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.106
Plasmid#184975PurposeExpress -Eco1 CAN1 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 12
ExpressionYeastMutationCAN1 donor G444stopPromoterGal7Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.111
Plasmid#184980PurposeTest effect of extending a1/a2 on TRP2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 27 v1
ExpressionYeastMutationTRP2 donor E64stop, a1/a2 length extended to 27 bpPromoterGal7Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.0
Plasmid#190795PurposePRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning processDepositorInsertPRE-Hsp70Bb-Cas9-T2A-eGFP
UseCRISPRExpressionInsectAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.037
Plasmid#184966PurposeExpress -Eco1 dead RT and ncRNA in yeasstDepositorInsertEco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT
ExpressionYeastMutationHuman codon optimized RT, YXDD catalytic region m…PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.112
Plasmid#184981PurposeExpress -Eco1 FAA1 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12
ExpressionYeastMutationFAA1 donor P233stopPromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.113
Plasmid#184982PurposeTest effect of extending a1/a2 on FAA1 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
ExpressionYeastMutationFAA1 donor P233stop, a1/a2 length extended to 27 …PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.002
Plasmid#184972PurposeExpress -Eco1 ADE2 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 12
ExpressionYeastMutationADE2 donor P272stopPromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.110
Plasmid#184979PurposeExpress -Eco1 TRP2 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12
ExpressionYeastMutationTRP2 donor E64stopPromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.039
Plasmid#184973PurposeTest effect of extending a1/a2 on ADE2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
ExpressionYeastMutationADE2 donor P272stop, a1/a2 length extended to 27 …PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
hsLEMD2-pX330
Plasmid#179511PurposeEncodes gRNA for human LEMD2DepositorInsertgRNA for LEMD2
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7
Plasmid#186753PurposeA basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.DepositorTypeEmpty backboneUseSynthetic Biology; Cloning backbonePromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-NLSlacZ-stop-L2
Plasmid#186358PurposeEntry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.DepositorInsertlacZ (lacZ Synthetic, E. coli)
UseExpression of nuclear lacz reporterTagsNuclear localization signalAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.407
Plasmid#187204PurposeExpresses retron Eco1 ncRNA v32, and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v32
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterT7/LacAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.408
Plasmid#187205PurposeExpresses retron Eco1 ncRNA v35, and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v35
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterT7/LacAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB438
Plasmid#185092PurposeGAL1::Rfa1[1-337, 344-621]-GFP with NLS removedDepositorInsertRFA1
ExpressionYeastMutationRfa1 with amino acids 338-343 deletedPromoterGAL1Available SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB372
Plasmid#185084PurposeGAL::Rfa1[330-621]-GFP under control of GAL1 promoterDepositorInsertRFA1
TagsGFPExpressionYeastMutationRfa1 amino acids 330-621PromoterGAL1Available SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)
Plasmid#158771PurposeExpression of synaptotagmin 1 variant with self-cleaving C2 domain.DepositorInsertSynaptotagmin 1 1-NS3/4a (Syt1 Mouse)
UseLentiviralMutationFlexible linkers, ADVVCC/QMSY cleavage siteAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
NCH2exp
Plasmid#131019PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH2
TagseGFP-FLAG-HAExpressionPlantPromoterP35SAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MNEAP1ec
Plasmid#131020PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMNEAP1
UseGateway entry vectorTagseGFP-FLAG-HAAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits