We narrowed to 31,693 results for: LIS;

1. pYL322d1/N-Vn

   Plasmid

   #183153

   Purpose

   Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vn.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-S in pAR9 (rep/cap)

   Plasmid

   #174539

   Purpose

   encodes AAV-S capsid for efficient gene delivery to the mammalian cochlea

   Depositor

   Casey Maguire

   Article

   Ivanchenko et al Mol Ther Methods Clin Dev. 2021 Mar 29;2

   Insert

   VP1 of AAV9 containing the AAV-S 7-mer peptide insert
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-C1-mTrim21ΔPRYSPRY

   Plasmid

   #105518

   Purpose

   Expression of mCherry-tagged mouse Trim21 with a deleted PRYSPRY domain in mammalian cells

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   deletion of PRYSPRY domain (aa1-281)

   Promoter

   CMV

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSH231-EF1-RFP-HYGRO

   Plasmid

   #115145

   Purpose

   Safe harbor site 231 knock-in vector with RFP-HygromycinR expression cassette

   Depositor

   Raymond Monnat, Michael Phelps

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   RFP
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCG150_double_phiC31_attB

   Plasmid

   #169693

   Purpose

   cargo vector for the phiC31 system with, unc-119 rescue fragment, no fluorescent marker. Gateway R3R4 sites (for gene of interest insertion)

   Depositor

   Shih-Peng Chan

   Article

   Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRubiC-T2A-Cas9

   Plasmid

   #75347

   Purpose

   Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   mCherry (via t2a)

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-BoxB

   Plasmid

   #92004

   Purpose

   Expresses firefly luciferase with BoxB sequences in 3' UTR

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   Firefly Luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag

   Plasmid

   #132402

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. 5'-BirA-FLAG GFP minimal CMV pCDNA5

   Plasmid

   #212087

   Purpose

   LR vector for integration of GFP into N2a FRT rtTA3 expression cells (BioID vector)

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   GFP
   

   Expression

   Mammalian

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYL322d1/Vn-C

    Plasmid

    #183154

    Purpose

    Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQAT42

    Plasmid

    #223414

    Purpose

    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-ecTadA8e-SpRY-D10A-ZmUbi-gRNA scaffold-tRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQAT21

    Plasmid

    #223393

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by 2x35s and the sgRNA was driven by AtU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-hA3A-Y130F-SpRYD10A-UGI-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CMV-Sa-SauriCas9

    Plasmid

    #135967

    Purpose

    Expresses Sa-SauriCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19/mChe-Golgi

    Plasmid

    #183169

    Purpose

    Construct for transient expression of Golgi apparatus-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    mCherry-Golgi
    

    Tags

    AtRER1B, A. thaliana endoplasmic reticulum retrie…

    Expression

    Plant

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag

    Plasmid

    #132399

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPguCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H151A; H1121A

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-HA-MyD88-FW/AA

    Plasmid

    #12289

    Depositor

    Bruce Beutler

    Article

    Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    MyD88 (MYD88 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F285A/W286A

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pYPQAT38

    Plasmid

    #223410

    Purpose

    T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQAT27

    Plasmid

    #223399

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by 2x35s and the sgRNA was driven by AtU3; Kanamycin for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQAT13

    Plasmid

    #223385

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU3-gRNA

    Plasmid

    #53063

    Purpose

    Transcription of guide RNA (gRNA) in rice cells

    Depositor

    Caixia Gao

    Article

    Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

    Insert

    OsU3 promoter + gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Promoter

    rice U3

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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